PMID- 17490538
OWN - NLM
STAT- MEDLINE
DCOM- 20071030
LR  - 20161018
IS  - 1009-2137 (Print)
IS  - 1009-2137 (Linking)
VI  - 15
IP  - 1
DP  - 2007 Feb
TI  - Ex vivo specific induction of CD8 positive anti-leukemia cytotoxic lymphocytes 
      from umbilical cord blood.
PG  - 129-33
AB  - Minimal residual disease (MRD) is the principal root of relapsed leukemia. 
      Application of specific immunotherapy is effective in eradication of MRD and one 
      of the immunotherapeutic strategies is induction and re-transfusion of 
      leukemia-specific cytotoxic T lymphocytes (CTLs). This study was aimed to 
      investigate the possibility of ex vivo induction and generation of CD8 positive 
      CTLs of umbilical cord blood cell origin and to explore their potential of 
      specific anti-leukemia cytotoxicity so as to evaluate the feasibility of 
      application of cord blood cell derived lymphocytes to specific immunotherapy. 
      Dendritic cells (DCs) were induced and generated ex vivo from cord blood 
      mononuclear cells (MNC) with a cytokine cocktail, and loaded with frozen and 
      thawed U937 leukemia antigen. The matured DCs were used to stimulate T 
      lymphocytes derived from the same cord blood cell sample into CTLs. CD8 positive 
      CTLs were then isolated by magnetic activated cell sorting (MACS). Inverted 
      microscopy, scanning electronic microscopy and flow cytometry were used to detect 
      DCs and methyl thiazolyl tetrazolium (MTT) cytotoxicity method was used to assay 
      the killing activity. The results showed that DCs with typical morphology and 
      mature function were cultured from 10 human cord blood cell samples. The 
      cytotoxicities of CD8 positive CTLs, CD8 negative CTLs and T lymphocytes (TLs) to 
      U937 cells were (66.36 +/- 12.43)%, (34.47 +/- 8.19)% and (15.79 +/- 4.64)% 
      respectively under the same effector target ratio (40:1). Among them, the 
      anti-leukemia cytotoxicity of the CD8 positive group was highest. At effctor 
      target ratio of 40 to 1, the cytotoxicity of CD8 positive CTLs to U937 cells 
      (66.36%) was higher than that to K562 cells (41.97%) (P < 0.05), whereas the 
      cytotoxicity of CD8 negative CTLs to U937 cells was not significantly different 
      from that to K562 cells (P > 0.05). It is concluded that specific CD8 positive 
      CTLs can be generated from cord blood lymphocytes by induction of mature cord 
      blood DCs loaded with U937 leukemia antigen. The cytotoxicity of CD8 positive 
      CTLs against U937 cell is more potent than CD8 negative CTLs, and is strain 
      specific.
FAU - Tan, Huo
AU  - Tan H
AD  - Centre of Oncology and Hematology, The First Affiliated Hospital of Guangzhou 
      Medical College, Guangzhou 510230, China. tanhuo@public.guangzhou.gd.cn
FAU - Liu, Xin
AU  - Liu X
FAU - Ye, Xu
AU  - Ye X
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhongguo Shi Yan Xue Ye Xue Za Zhi
JT  - Zhongguo shi yan xue ye xue za zhi
JID - 101084424
RN  - 0 (Antigens, Neoplasm)
SB  - IM
MH  - Antibody Specificity
MH  - Antigens, Neoplasm/immunology
MH  - CD8-Positive T-Lymphocytes/*immunology
MH  - Cells, Cultured
MH  - Dendritic Cells/immunology
MH  - Fetal Blood/*cytology/immunology
MH  - Humans
MH  - Immunotherapy
MH  - T-Cell Antigen Receptor Specificity
MH  - T-Lymphocytes, Cytotoxic/*immunology
MH  - U937 Cells
EDAT- 2007/05/11 09:00
MHDA- 2007/10/31 09:00
CRDT- 2007/05/11 09:00
PHST- 2007/05/11 09:00 [pubmed]
PHST- 2007/10/31 09:00 [medline]
PHST- 2007/05/11 09:00 [entrez]
AID - 1009-2137(2007)01-0129-05 [pii]
PST - ppublish
SO  - Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2007 Feb;15(1):129-33.