PMID- 17490782
OWN - NLM
STAT- MEDLINE
DCOM- 20080721
LR  - 20200930
IS  - 0176-1617 (Print)
IS  - 0176-1617 (Linking)
VI  - 165
IP  - 5
DP  - 2008
TI  - Intron splicing in 5' untranslated region of the rolA transcript in transgenic 
      apple.
PG  - 544-52
AB  - The rolA gene encoded on the Ri plasmid of Agrobacterium rhizogenes causes 
      developmental alterations, including dwarfing characteristics in the transgenic 
      plants. In an attempt to introduce dwarfing characteristics into apple rootstocks 
      for breeding purposes, the rolA gene was incorporated into the apple rootstock 
      M26 and obtained four transgenic clones. All the clones exhibited reduced growth 
      compared to untransformed control plants but different degree of dwarfing and 
      wrinkled leaves. In the present study, expression of the rolA gene was further 
      investigated by analysing the structure of the rolA transcript and the levels of 
      the rolA mRNAs from these clones. The nucleotide (nt) sequence of the rolA 
      transcript showed two forms of the transcript: one, the unspliced form, was 
      co-linear with the rolA sequence in the genomic DNA; the other was spliced mRNA 
      in which an 85-base pair (bp) intron sequence in the 5' untranslated region 
      (5'UTR) was spliced out. The position of splicing is different from that in 
      Arabidopsis thaliana but similar to the splicing site found in tobacco. The 
      transcription start region of the rolA gene in apple was 206bp upstream of that 
      in Arabidopsis and 277bp upstream to Nicotiana tabacum transcription start. A 
      hairpin-like secondary structure and an upstream open reading frame (uORF) were 
      revealed in the rolA 5'UTR. The levels of the rolA mRNA in the apple transgenic 
      clones were analysed by semi-quantitative reverse transcription-polymerase chain 
      reaction (RT-PCR). The results showed slight variation in the shoot tissues of 
      the transgenic clones.
FAU - Xue, Zhong-Tian
AU  - Xue ZT
AD  - Department of Crop Science, The Swedish University of Agricultural Sciences, PO 
      Box 55, S-230 53 Alnarp, Sweden.
FAU - Holefors, Anna
AU  - Holefors A
FAU - Welander, Margareta
AU  - Welander M
LA  - eng
SI  - GENBANK/AF442734
SI  - GENBANK/AF442735
SI  - GENBANK/AF442736
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20070508
PL  - Germany
TA  - J Plant Physiol
JT  - Journal of plant physiology
JID - 9882059
RN  - 0 (Bacterial Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Untranslated Regions)
RN  - 0 (rolA protein, Agrobacterium rhizogenes)
SB  - IM
MH  - Bacterial Proteins/*genetics
MH  - Base Sequence
MH  - Introns/*genetics
MH  - Malus/*genetics
MH  - Molecular Sequence Data
MH  - Plants, Genetically Modified/*genetics
MH  - *RNA Splicing
MH  - RNA, Messenger/genetics/metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Sequence Homology, Nucleic Acid
MH  - Transcription, Genetic
MH  - Untranslated Regions/*genetics
EDAT- 2007/05/11 09:00
MHDA- 2008/07/22 09:00
CRDT- 2007/05/11 09:00
PHST- 2006/10/02 00:00 [received]
PHST- 2006/11/21 00:00 [revised]
PHST- 2006/11/24 00:00 [accepted]
PHST- 2007/05/11 09:00 [pubmed]
PHST- 2008/07/22 09:00 [medline]
PHST- 2007/05/11 09:00 [entrez]
AID - S0176-1617(07)00039-9 [pii]
AID - 10.1016/j.jplph.2006.11.010 [doi]
PST - ppublish
SO  - J Plant Physiol. 2008;165(5):544-52. doi: 10.1016/j.jplph.2006.11.010. Epub 2007 
      May 8.