PMID- 17492792
OWN - NLM
STAT- MEDLINE
DCOM- 20070921
LR  - 20220309
IS  - 0360-4012 (Print)
IS  - 0360-4012 (Linking)
VI  - 85
IP  - 9
DP  - 2007 Jul
TI  - Developmental changes in the association of NMDA receptors with lipid rafts.
PG  - 1876-83
AB  - Lipid rafts (LR) are lipid microdomains present in the cell surface membrane that 
      are organizational platforms involved in protein trafficking and formation of 
      cell signaling complexes. In the adult brain, NMDA receptors (NMDAR) and 
      receptor-associated proteins such as membrane-associated guanylate kinases 
      (PSD-95 and SAP102), are distributed between the postsynaptic density (PSD) and 
      lipid rafts. However, the time course of the association of NMDAR with LR during 
      neural development is not known. We therefore investigated the effect of 
      development on the association of NMDAR with LR prepared from rat brains ranging 
      in postnatal age from 1-35 days and compared this with their expression in PSDs. 
      LR and PSD fractions were prepared by extraction of P2 membranes with Tx-100 
      followed by sucrose density gradient centrifugation. The yield of LR, as 
      reflected by levels of protein, Thy-1, and flotillin-1 increased during postnatal 
      development. NR2A was associated predominantly with the lipid raft fraction at 
      all ages examined whereas NR2B underwent a gradual shift from PSDs to lipid rafts 
      during the first 3 weeks after birth. These changes in the distribution of NR2A 
      and NR2B were paralleled by changes in the distribution of PSD-95 and SAP102 
      respectively. Tyrosine-phosphorylated proteins, including NR2A and NR2B, were 
      preferentially associated with lipid rafts in older, as compared to younger, 
      animals. These results show that the association of NMDAR with LR is regulated 
      developmentally.
FAU - Besshoh, Shintaro
AU  - Besshoh S
AD  - Centre for the Neurobiology of Stress, Department of Life Sciences, University of 
      Toronto Scarborough, Toronto, Canada.
FAU - Chen, Sheng
AU  - Chen S
FAU - Brown, Ian R
AU  - Brown IR
FAU - Gurd, James W
AU  - Gurd JW
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Neurosci Res
JT  - Journal of neuroscience research
JID - 7600111
RN  - 0 (Disks Large Homolog 4 Protein)
RN  - 0 (Dlg3 protein, rat)
RN  - 0 (Dlg4 protein, rat)
RN  - 0 (Intracellular Signaling Peptides and Proteins)
RN  - 0 (Membrane Proteins)
RN  - 0 (NR2A NMDA receptor)
RN  - 0 (NR2B NMDA receptor)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Neuropeptides)
RN  - 0 (Receptors, N-Methyl-D-Aspartate)
RN  - 42HK56048U (Tyrosine)
RN  - 97C5T2UQ7J (Cholesterol)
SB  - IM
MH  - Aging/metabolism
MH  - Animals
MH  - Cholesterol/metabolism
MH  - Disks Large Homolog 4 Protein
MH  - Electrophoresis, Polyacrylamide Gel
MH  - Immunoblotting
MH  - Intracellular Signaling Peptides and Proteins/metabolism
MH  - Membrane Microdomains/*metabolism
MH  - Membrane Proteins/metabolism
MH  - Nerve Tissue Proteins/metabolism
MH  - Neuropeptides/metabolism
MH  - Phosphorylation
MH  - Rats
MH  - Receptors, N-Methyl-D-Aspartate/*metabolism
MH  - Tyrosine/metabolism
EDAT- 2007/05/12 09:00
MHDA- 2007/09/22 09:00
CRDT- 2007/05/12 09:00
PHST- 2007/05/12 09:00 [pubmed]
PHST- 2007/09/22 09:00 [medline]
PHST- 2007/05/12 09:00 [entrez]
AID - 10.1002/jnr.21336 [doi]
PST - ppublish
SO  - J Neurosci Res. 2007 Jul;85(9):1876-83. doi: 10.1002/jnr.21336.