PMID- 17493347 OWN - NLM STAT- MEDLINE DCOM- 20071030 LR - 20221207 IS - 1009-2137 (Print) IS - 1009-2137 (Linking) VI - 15 IP - 2 DP - 2007 Apr TI - [Gene and haplotype frequencies for the loci HLA-A, B and DRB1 in 11755 north Chinese Han bone marrow registry donors]. PG - 357-63 AB - The study was aimed to investigate the human leukocyte antigen (HLA)-A, B, DRB1 alleles and haplotype frequencies and the characteristics of linkage disequilibrium in north Chinese Han bone marrow donors. HLA phenotype data of 11 755 north Chinese Han bone marrow donors were identified by PCR-SSP and PCR-SSO. HLA-A, B, DRB1 allele and haplotype frequencies were calculated by computer software named Arleguin which was based on Expectation-Maximization (EM) algorithms. The results showed that the population of 11755 unrelated-donors was tested by Hardy-Weinberg equilibrium, and 18,42 and 15 specificities of HLA alleles were identified on the HLA-A, B, DRB1 locus respectively, including HLA-A25, B42, B53, B73 and DR3 which were rarely reported in Han population. HLA-A36, A43, A80, B78, B82 and DR18 were not detected in this study. The most frequent alleles with a frequency of over 0.05 were HLA-A*02, A*11, A*24, A*33, A*30, A*01, A*03, A*13, B62, B*51, B*46, B60, B61, B*35, B*44, DRB1*15, DRB1*09, DRB1*04, DRB1*07, DRB1*12, DRB1*11, DRB1*14, DRB1*08, DRB1*13. There were a total of 2 026 kinds of HLA-A-B-DR haplotypes (with a frequency of over 10(-6)) to be obtained. The each frequency of 26 kinds of three-locus haplotypes including HLA-A30-B13-DR7, A2-B46-DR9, A33-B58-DR17 etc was higher than 0.005. A30-B13-DR7 was the most frequent haplotype in north Chinese Han population. There were a total of 538 kinds of haplotypes for HLA-A-B, 227 kinds for A-DR and 522 kinds for B-DR to be obtained, and there were 409, 195, 423 kinds of haplotypes respectively with a frequency higher than 10 - 6. There were 28 kinds of HLA-A-B haplotypes including A30-B13, A2-B46, A33-B58 etc, 26 kinds of HLA-A-DR haplotypes including A2-DR9, A2-DR15, A30-DR7 etc, and 24 kinds of HLA-B-DR haplotypes including B13-DR7, B46-DR9, B13-DR12 etc with a frequency higher than 0.01. 296 (72%) kinds of HLA-A-B, 130 (67%) kinds of A-DR and 308 (73%) kinds of B-DR haplotypes were statistical linkage disequilibrium. HLA-A30-B13, A33-B58, A1-B37, A30-DR7, A33-DR13, A1-DR10, B37-DR10, B8-DR17, B13-DR7, B58-DR17 were significant positive linkage disequilibrium. It is concluded that this HLA-A, B, DRB1 gene and haplotype frequencies and linkage disequilibrium data with the largest sample size up to now is unique in north Chinese Han population. The study will be helpful to find matched donors for patients and establish the important foundation for further studying of transplantation immunity, HLA-related diseases and population genetics of this area. FAU - Wu, Qiang-Ju AU - Wu QJ AD - Blood Center of Shanxi Province, Xi'an 710061, China. FAU - Liu, Meng-Li AU - Liu ML FAU - Qi, Jun AU - Qi J FAU - Liu, Sheng AU - Liu S FAU - Zhang, Yan AU - Zhang Y FAU - Wei, Xiao-Qian AU - Wei XQ LA - chi PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - China TA - Zhongguo Shi Yan Xue Ye Xue Za Zhi JT - Zhongguo shi yan xue ye xue za zhi JID - 101084424 RN - 0 (HLA-A Antigens) RN - 0 (HLA-B Antigens) RN - 0 (HLA-DR Antigens) RN - 0 (HLA-DRB1 Chains) SB - IM MH - Adolescent MH - Adult MH - Alleles MH - Asian People/genetics MH - *Blood Donors MH - Female MH - Gene Frequency MH - HLA-A Antigens/*genetics MH - HLA-B Antigens/*genetics MH - HLA-DR Antigens/*genetics MH - HLA-DRB1 Chains MH - *Haplotypes MH - Humans MH - Male MH - Middle Aged MH - Registries EDAT- 2007/05/12 09:00 MHDA- 2007/10/31 09:00 CRDT- 2007/05/12 09:00 PHST- 2007/05/12 09:00 [pubmed] PHST- 2007/10/31 09:00 [medline] PHST- 2007/05/12 09:00 [entrez] AID - 1009-2137(2007)02-0357-07 [pii] PST - ppublish SO - Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2007 Apr;15(2):357-63.