PMID- 17494075
OWN - NLM
STAT- MEDLINE
DCOM- 20070914
LR  - 20220331
IS  - 0022-538X (Print)
IS  - 1098-5514 (Electronic)
IS  - 0022-538X (Linking)
VI  - 81
IP  - 14
DP  - 2007 Jul
TI  - CD163 expression confers susceptibility to porcine reproductive and respiratory 
      syndrome viruses.
PG  - 7371-9
AB  - Direct functional screening of a cDNA expression library derived from primary 
      porcine alveolar macrophages (PAM) revealed that CD163 is capable of conferring a 
      porcine reproductive and respiratory syndrome virus (PRRSV)-permissive phenotype 
      when introduced into nonpermissive cells. Transient-transfection experiments 
      showed that full-length CD163 cDNAs from PAM, human U937 cells (histiocytic 
      lymphoma), African green monkey kidney cells (MARC-145 and Vero), primary mouse 
      peritoneal macrophages, and canine DH82 (histocytosis) cells encode functional 
      virus receptors. In contrast, CD163 splice variants without the C-terminal 
      transmembrane anchor domain do not provide PRRSV receptor function. We 
      established several stable cell lines expressing CD163 cDNAs from pig, human, and 
      monkey, using porcine kidney (PK 032495), feline kidney (NLFK), or baby hamster 
      kidney (BHK-21) as the parental cell lines. These stable cell lines were 
      susceptible to PRRSV infection and yielded high titers of progeny virus. Cell 
      lines were phenotypically stable over 80 cell passages, and PRRSV could be 
      serially passed at least 60 times, yielding in excess of 10(5) 50% tissue culture 
      infective doses/ml.
FAU - Calvert, Jay G
AU  - Calvert JG
AD  - Veterinary Medicine Research and Development, Animal Health Division, Pfizer 
      Incl., Kalamazoo, MI 49001, USA. jay.calvert@pfizer.com
FAU - Slade, David E
AU  - Slade DE
FAU - Shields, Shelly L
AU  - Shields SL
FAU - Jolie, Rika
AU  - Jolie R
FAU - Mannan, Ramasamy M
AU  - Mannan RM
FAU - Ankenbauer, Robert G
AU  - Ankenbauer RG
FAU - Welch, Siao-Kun W
AU  - Welch SK
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20070509
PL  - United States
TA  - J Virol
JT  - Journal of virology
JID - 0113724
RN  - 0 (Antigens, CD)
RN  - 0 (Antigens, Differentiation, Myelomonocytic)
RN  - 0 (CD163 antigen)
RN  - 0 (DNA Primers)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Cell Surface)
RN  - 0 (Receptors, Immunologic)
RN  - 0 (SIGLEC1 protein, human)
RN  - 0 (Sialic Acid Binding Ig-like Lectin 1)
RN  - 0 (Siglec1 protein, mouse)
SB  - IM
MH  - Animals
MH  - Antigens, CD/genetics/*immunology
MH  - Antigens, Differentiation, Myelomonocytic/genetics/*immunology
MH  - Base Sequence
MH  - Cell Line
MH  - Cloning, Molecular
MH  - DNA Primers
MH  - Disease Susceptibility/*immunology
MH  - Genotype
MH  - Humans
MH  - Membrane Glycoproteins/genetics
MH  - Porcine Reproductive and Respiratory Syndrome/immunology/prevention & control
MH  - Porcine respiratory and reproductive syndrome virus/*immunology/physiology
MH  - RNA, Messenger/genetics
MH  - Receptors, Cell Surface/genetics/*immunology
MH  - Receptors, Immunologic/genetics
MH  - Sialic Acid Binding Ig-like Lectin 1
MH  - Swine
MH  - Transfection
MH  - Virus Replication
PMC - PMC1933360
EDAT- 2007/05/12 09:00
MHDA- 2007/09/15 09:00
PMCR- 2007/11/01
CRDT- 2007/05/12 09:00
PHST- 2007/05/12 09:00 [pubmed]
PHST- 2007/09/15 09:00 [medline]
PHST- 2007/05/12 09:00 [entrez]
PHST- 2007/11/01 00:00 [pmc-release]
AID - JVI.00513-07 [pii]
AID - 0513-07 [pii]
AID - 10.1128/JVI.00513-07 [doi]
PST - ppublish
SO  - J Virol. 2007 Jul;81(14):7371-9. doi: 10.1128/JVI.00513-07. Epub 2007 May 9.