PMID- 17494076
OWN - NLM
STAT- MEDLINE
DCOM- 20070914
LR  - 20181113
IS  - 0022-538X (Print)
IS  - 1098-5514 (Electronic)
IS  - 0022-538X (Linking)
VI  - 81
IP  - 14
DP  - 2007 Jul
TI  - Human immunodeficiency virus type 1 replication in dendritic cell-T-cell 
      cocultures is increased upon incorporation of host LFA-1 due to higher levels of 
      virus production in immature dendritic cells.
PG  - 7672-82
AB  - Dendritic cells (DCs) act as a portal for invasion by human immunodeficiency 
      virus type-1 (HIV-1). Here, we investigated whether virion-incorporated host cell 
      membrane proteins can affect virus replication in DC-T-cell cocultures. Using 
      isogenic viruses either devoid of or bearing host-derived leukocyte 
      function-associated antigen 1 (LFA-1), we showed that HIV-1 production is 
      augmented when LFA-1-bearing virions are used compared to that for viral entities 
      lacking this adhesion molecule. This phenomenon was observed in immature 
      monocyte-derived DCs (IM-MDDCs) only and not in DCs displaying a mature 
      phenotype. The increase is not due to higher virus production in responder CD4(+) 
      T cells but rather is linked with a more important productive infection of 
      IM-MDDCs. We provided evidence that virus-associated host LFA-1 molecules do not 
      affect a late event in the HIV-1 life cycle but rather exert an effect on an 
      early step in virus replication. We demonstrated that the enhancement of 
      productive infection of IM-MDDCs that is conferred by virus-anchored host LFA-1 
      involves the protein kinase A (PKA) and PKC signal transduction pathways. The 
      biological significance of this phenomenon was established by performing 
      experiments with virus stocks produced in primary human cells and anti-LFA-1 
      antibodies. Together, our results indicate that the association between some 
      virus-bound host proteins and their natural cognate ligands can modulate de novo 
      HIV-1 production by IM-MDDCs. Therefore, the additional interactions between 
      virus-bound host cell membrane constituents and counter receptors on the surfaces 
      of DCs can influence HIV-1 replication in IM-MDDC-T-cell cocultures.
FAU - Gilbert, Caroline
AU  - Gilbert C
AD  - Research Center in Infectious Diseases, Laval Univeristy, Quebec, Canada.
FAU - Cantin, Rejean
AU  - Cantin R
FAU - Barat, Corinne
AU  - Barat C
FAU - Tremblay, Michel J
AU  - Tremblay MJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20070509
PL  - United States
TA  - J Virol
JT  - Journal of virology
JID - 0113724
RN  - 0 (DNA Primers)
RN  - 0 (Lymphocyte Function-Associated Antigen-1)
RN  - EC 2.7.11.11 (Cyclic AMP-Dependent Protein Kinases)
RN  - EC 2.7.11.13 (Protein Kinase C)
SB  - IM
MH  - Base Sequence
MH  - Coculture Techniques
MH  - Cyclic AMP-Dependent Protein Kinases/metabolism
MH  - DNA Primers
MH  - Dendritic Cells/*virology
MH  - Disease Reservoirs
MH  - HIV-1/*physiology
MH  - Humans
MH  - Lymphocyte Function-Associated Antigen-1/*physiology
MH  - Polymerase Chain Reaction
MH  - Protein Kinase C/metabolism
MH  - Signal Transduction
MH  - Virus Replication/*physiology
PMC - PMC1933380
EDAT- 2007/05/12 09:00
MHDA- 2007/09/15 09:00
PMCR- 2007/11/01
CRDT- 2007/05/12 09:00
PHST- 2007/05/12 09:00 [pubmed]
PHST- 2007/09/15 09:00 [medline]
PHST- 2007/05/12 09:00 [entrez]
PHST- 2007/11/01 00:00 [pmc-release]
AID - JVI.02810-06 [pii]
AID - 2810-06 [pii]
AID - 10.1128/JVI.02810-06 [doi]
PST - ppublish
SO  - J Virol. 2007 Jul;81(14):7672-82. doi: 10.1128/JVI.02810-06. Epub 2007 May 9.