PMID- 17495108
OWN - NLM
STAT- MEDLINE
DCOM- 20070926
LR  - 20191210
IS  - 1066-5099 (Print)
IS  - 1066-5099 (Linking)
VI  - 25
IP  - 8
DP  - 2007 Aug
TI  - Extensive studies on perfusion method plus intra-bone marrow-bone marrow 
      transplantation using cynomolgus monkeys.
PG  - 2098-103
AB  - The collection of bone marrow cells (BMCs) using a perfusion method has been 
      advantageous not only because of the low contamination of BMCs with T cells from 
      the peripheral blood but also the enrichment of stromal cells, which support 
      hemopoiesis. Before the application of this new method to humans, its safety 
      needed to be confirmed using cynomolgus monkeys. We therefore performed the 
      perfusion method on more than 100 cynomolgus monkeys using the long bones (such 
      as the humerus and femur) and also the iliac bones (for human application); in 
      the more than 150 trials to date, there have been no accidental deaths. 
      Furthermore, the technical safety of a new method for the intra-bone marrow (IBM) 
      injection of BMCs (termed IBM-bone marrow transplantation) has also been 
      confirmed using 30 monkeys. Disclosure of potential conflicts of interest is 
      found at the end of this article.
FAU - Inaba, Muneo
AU  - Inaba M
AD  - First Department of Pathology, Kansai Medical University, Moriguchi City, Osaka, 
      Japan.
FAU - Adachi, Yasushi
AU  - Adachi Y
FAU - Hisha, Hiroko
AU  - Hisha H
FAU - Hosaka, Naoki
AU  - Hosaka N
FAU - Maki, Masahiko
AU  - Maki M
FAU - Ueda, Yusuke
AU  - Ueda Y
FAU - Koike, Yasushi
AU  - Koike Y
FAU - Miyake, Takeshi
AU  - Miyake T
FAU - Fukui, Junichi
AU  - Fukui J
FAU - Cui, Yunze
AU  - Cui Y
FAU - Mukaide, Hiromi
AU  - Mukaide H
FAU - Koike, Naoko
AU  - Koike N
FAU - Omae, Mariko
AU  - Omae M
FAU - Mizokami, Tomomi
AU  - Mizokami T
FAU - Shigematsu, Akio
AU  - Shigematsu A
FAU - Sakaguchi, Yutaku
AU  - Sakaguchi Y
FAU - Tsuda, Masanobu
AU  - Tsuda M
FAU - Okazaki, Satoshi
AU  - Okazaki S
FAU - Wang, Xiaoli
AU  - Wang X
FAU - Li, Qing
AU  - Li Q
FAU - Nishida, Akihisa
AU  - Nishida A
FAU - Ando, Yugo
AU  - Ando Y
FAU - Guo, Kequan
AU  - Guo K
FAU - Song, Changye
AU  - Song C
FAU - Cui, Wenhao
AU  - Cui W
FAU - Feng, Wei
AU  - Feng W
FAU - Katou, Junko
AU  - Katou J
FAU - Sado, Katuyuki
AU  - Sado K
FAU - Nakamura, Shuji
AU  - Nakamura S
FAU - Ikehara, Susumu
AU  - Ikehara S
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20070510
PL  - England
TA  - Stem Cells
JT  - Stem cells (Dayton, Ohio)
JID - 9304532
SB  - IM
MH  - Animals
MH  - Bone Marrow Cells/*cytology
MH  - Bone Marrow Transplantation/adverse effects/*methods
MH  - Cell Count
MH  - Cell Separation
MH  - Macaca fascicularis
MH  - Perfusion/*methods
MH  - Tissue and Organ Harvesting/adverse effects/instrumentation/*methods
MH  - Transplantation, Homologous
EDAT- 2007/05/15 09:00
MHDA- 2007/09/27 09:00
CRDT- 2007/05/15 09:00
PHST- 2007/05/15 09:00 [pubmed]
PHST- 2007/09/27 09:00 [medline]
PHST- 2007/05/15 09:00 [entrez]
AID - 2007-0174 [pii]
AID - 10.1634/stemcells.2007-0174 [doi]
PST - ppublish
SO  - Stem Cells. 2007 Aug;25(8):2098-103. doi: 10.1634/stemcells.2007-0174. Epub 2007 
      May 10.