PMID- 17495399
OWN - NLM
STAT- MEDLINE
DCOM- 20070717
LR  - 20190827
IS  - 0910-6340 (Print)
IS  - 0910-6340 (Linking)
VI  - 23
IP  - 5
DP  - 2007 May
TI  - Optimization of critical factors affecting the performance of an allergen chip 
      for the analysis of an allergen-specific human IgE in serum.
PG  - 545-9
AB  - A sensitive and multiplexed assay of allergen-specific human immunoglobulin E 
      (IgE) is of great significance in the precise diagnosis of allergies. We report 
      on the optimization of critical factors for chip-based analysis of IgE in human 
      serum with a high reliability. Extracts of two mite species were used as model 
      allergens, and were spotted onto a glass slide for the construction of an 
      allergen chip. Respective allergen-specific IgE in human serum was analyzed by 
      using biotinylated anti-human IgE and a streptavidin-Cy3 conjugate. Factors 
      affecting the performance of the allergen chip were investigated and optimized. 
      Especially, the effect of additives, the concentrations of biotinylated 
      anti-human IgE and the streptavidin-Cy3 conjugate, the serum dilution factor, and 
      the concentration of allergen extract as a capturing agent were examined in 
      detail. Under the optimized conditions, a chip-based analysis for sera from 43 
      patients revealed a reliable and reproducible diagnosis of respective allergies, 
      showing a good correlation with a conventional CAP assay.
FAU - Han, Min-Kyu
AU  - Han MK
AD  - Department of Biological Sciences, Korea Advanced Institute of Science and 
      Technology, Korea.
FAU - Seo, Moon-Hyeong
AU  - Seo MH
FAU - Lee, Dohoon
AU  - Lee D
FAU - Kim, Seung Hyun
AU  - Kim SH
FAU - Park, Hae-Sim
AU  - Park HS
FAU - Kim, Hak-Sung
AU  - Kim HS
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Switzerland
TA  - Anal Sci
JT  - Analytical sciences : the international journal of the Japan Society for 
      Analytical Chemistry
JID - 8511078
RN  - 0 (Allergens)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Allergens/*immunology
MH  - Animals
MH  - *Antibody Specificity
MH  - Fluorescent Antibody Technique/*methods
MH  - Humans
MH  - Immunoglobulin E/*blood/*immunology
MH  - Protein Array Analysis/*methods
MH  - Pyroglyphidae/immunology
MH  - Sensitivity and Specificity
MH  - Species Specificity
EDAT- 2007/05/15 09:00
MHDA- 2007/07/18 09:00
CRDT- 2007/05/15 09:00
PHST- 2007/05/15 09:00 [pubmed]
PHST- 2007/07/18 09:00 [medline]
PHST- 2007/05/15 09:00 [entrez]
AID - JST.JSTAGE/analsci/23.545 [pii]
AID - 10.2116/analsci.23.545 [doi]
PST - ppublish
SO  - Anal Sci. 2007 May;23(5):545-9. doi: 10.2116/analsci.23.545.