PMID- 17496270
OWN - NLM
STAT- MEDLINE
DCOM- 20071017
LR  - 20210217
IS  - 0022-2275 (Print)
IS  - 0022-2275 (Linking)
VI  - 48
IP  - 8
DP  - 2007 Aug
TI  - CLA differently regulates adipogenesis in stromal vascular cells from porcine 
      subcutaneous adipose and skeletal muscle.
PG  - 1701-9
AB  - Conjugated linoleic acid (CLA), a mixture of isomers of linoleic acid, has 
      previously been shown to be able to decrease porcine subcutaneous (SC) adipose 
      tissue levels while increasing the count of intramuscular (IM) adipose tissue in 
      vivo. However, the underlying mechanisms through which it acts are poorly 
      understood. The objective of this study was to investigate the different effects 
      of CLA on adipogenesis in cultured SC adipose tissue and IM stromal vascular 
      cells obtained from neonatal pigs. As shown here, trans-10, cis-12 CLA decreased 
      the expression of adipocyte-specific genes as well as adipose precursor cell 
      numbers and the accumulation of lipid in cultured SC adipose tissue stromal 
      vascular cells. However, the cis-9, trans-11 CLA did not alter adipogenesis in SC 
      cultures. On the other hand, both CLA isomers increased the expression of 
      adipocyte-specific genes in IM cultures, together with the increasing 
      accumulation of lipid and Oil Red O-stained cells. Collectively, these data show 
      that CLA decreases SC adipose tissue but increases IM adipose tissue by different 
      regulation of adipocyte-specific gene expression. These results suggest that 
      adipogenesis in IM adipocytes differs from that in SC adipocytes.
FAU - Zhou, Xuan
AU  - Zhou X
AD  - National Key Laboratory of Animal Nutrition, China Agricultural University, 
      Beijing 100094, China.
FAU - Li, Defa
AU  - Li D
FAU - Yin, Jingdong
AU  - Yin J
FAU - Ni, Jianjun
AU  - Ni J
FAU - Dong, Bing
AU  - Dong B
FAU - Zhang, Jinxiao
AU  - Zhang J
FAU - Du, Min
AU  - Du M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20070511
PL  - United States
TA  - J Lipid Res
JT  - Journal of lipid research
JID - 0376606
RN  - 0 (Linoleic Acids, Conjugated)
SB  - IM
MH  - *Adipogenesis/genetics
MH  - Animals
MH  - Cell Proliferation
MH  - Cells, Cultured
MH  - Gene Expression Regulation
MH  - Immunohistochemistry
MH  - Linoleic Acids, Conjugated/*pharmacology
MH  - Muscle, Skeletal/blood supply/*cytology/metabolism
MH  - Stromal Cells/metabolism
MH  - Subcutaneous Fat/blood supply/*cytology/metabolism
MH  - Swine
EDAT- 2007/05/15 09:00
MHDA- 2007/10/18 09:00
CRDT- 2007/05/15 09:00
PHST- 2007/05/15 09:00 [pubmed]
PHST- 2007/10/18 09:00 [medline]
PHST- 2007/05/15 09:00 [entrez]
AID - S0022-2275(20)42506-4 [pii]
AID - 10.1194/jlr.M600525-JLR200 [doi]
PST - ppublish
SO  - J Lipid Res. 2007 Aug;48(8):1701-9. doi: 10.1194/jlr.M600525-JLR200. Epub 2007 
      May 11.