PMID- 17497413
OWN - NLM
STAT- MEDLINE
DCOM- 20070517
LR  - 20070514
IS  - 1528-7394 (Print)
IS  - 0098-4108 (Linking)
VI  - 70
IP  - 12
DP  - 2007 Jun
TI  - Increased neurotrophin production in a Penicillium chrysogenum-induced allergic 
      asthma model in mice.
PG  - 1020-6
AB  - Neurotrophins, including nerve growth factor (NGF), brain-derived neurotrophic 
      factor (BDNF), and neurotrophin (NT)-3, have been implicated in the pathogenesis 
      of many features and symptoms of asthma. The role of neurotrophins in fungal 
      allergic asthma, however, is unknown. Repeated pulmonary challenge with 
      Penicillium chrysogenum extract (PCE) induces dose-dependent allergic asthma-like 
      responses in mice. The aim of this study was to investigate whether neurotrophins 
      are involved in the PCE-induced allergic airway response in mice. Mice were 
      exposed to 10, 20, 50, or 70 microg PCE by involuntary aspiration 4 times over 1 
      mo. Bronchial alveolar lavage fluid (BALF) was collected immediately before and 
      after the final exposure. The levels of NGF, NT-3, and NT-4 were determined by 
      enzyme-linked immunosorbent assay (ELISA). The lungs were fixed and processed for 
      immunohistochemical examination of NGF production. PCE-exposed mice had 
      dose-dependent increases in NGF, NT-3, and NT-4 in both BALF and sera. Exposures 
      to PCE produced elevation in positive immunohistochemical staining for NGF in the 
      airway epithelium and smooth muscle cells, in addition to infiltrated cells such 
      as mononuclear cells, eosinophils, and macrophages. Taken together, this is the 
      first study to link fungal allergic asthma in an experimental model with enhanced 
      production of neurotrophins in the airways, and suggests that neurotrophins may 
      play a role in the etiology of mold-induced asthma in humans.
FAU - Chung, Yong Joo
AU  - Chung YJ
AD  - National Health and Environmental Effects Research Laboratory, U.S. Environmental 
      Protection Agency, Research Triangle Park, North Carolina, USA.
FAU - Farraj, Aimen
AU  - Farraj A
FAU - Coates, Najwa Haykal
AU  - Coates NH
FAU - Gavett, Stephen H
AU  - Gavett SH
FAU - Ward, Marsha D W
AU  - Ward MD
LA  - eng
PT  - Journal Article
PL  - England
TA  - J Toxicol Environ Health A
JT  - Journal of toxicology and environmental health. Part A
JID - 100960995
RN  - 0 (Nerve Growth Factors)
SB  - IM
MH  - Animals
MH  - Asthma/*immunology/*metabolism/microbiology
MH  - Bronchoalveolar Lavage Fluid/chemistry
MH  - Disease Models, Animal
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Female
MH  - Hypersensitivity/immunology
MH  - Immunohistochemistry
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Nerve Growth Factors/*metabolism
MH  - Penicillium chrysogenum/*pathogenicity
EDAT- 2007/05/15 09:00
MHDA- 2007/05/18 09:00
CRDT- 2007/05/15 09:00
PHST- 2007/05/15 09:00 [pubmed]
PHST- 2007/05/18 09:00 [medline]
PHST- 2007/05/15 09:00 [entrez]
AID - 778482981 [pii]
AID - 10.1080/15287390601172023 [doi]
PST - ppublish
SO  - J Toxicol Environ Health A. 2007 Jun;70(12):1020-6. doi: 
      10.1080/15287390601172023.