PMID- 17498491
OWN - NLM
STAT- MEDLINE
DCOM- 20080414
LR  - 20190922
IS  - 1672-1977 (Print)
IS  - 1672-1977 (Linking)
VI  - 5
IP  - 3
DP  - 2007 May
TI  - [Effects of Jianpi Huoxue Decoction on Kupffer cell signal pathway activation in 
      rats with liver injury induced by Lieber-Decarli liquid diet and 
      lipopolysaccharide].
PG  - 302-6
AB  - OBJECTIVE: To observe the effects of Jianpi Huoxue Decoction, a compound Chinese 
      herbal medicine, on Kupffer cell signal pathway activation in rats with liver 
      injury induced by Lieber-Decarli liquid diet and lipopolysaccharide (LPS). 
      METHODS: SD rats were divided into normal, control liquid diet, ethanol liquid 
      diet and ethanol liquid diet plus Jianpi Huoxue Decoction group. Rats were 
      administrated with Jianpi Huoxue Decoction or distilled water via gastrogavage 
      for 4 weeks after administration with ethanol or control liquid diet for 2 weeks 
      respectively. After that, rats in each group were stimulated with LPS via 
      gastrogavage for 3.5 h and harvested. Alanine aminotransferase (ALT) in serum and 
      triglyceride (TG) in liver were analyzed. Pathological changes in liver tissues 
      were observed in HE staining section. Tumor necrosis factor-alpha(TNF-alpha) in 
      portal vein plasma was assayed by ELISA. The protein expressions of CD68, 
      Toll-like receptor 4 (TLR4), phosphorylation-I kappa B (P-I kappa B) and 
      TNF-alpha in liver were evaluated with Western-blotting. RESULTS: After the 
      treatment with Jianpi Huoxue Decoction, the pathologic changes in liver tissue 
      were lightened, levels of ALT in serum, TG in liver and TNF-alpha in portal vein 
      plasma were decreased, and the protein expressions of CD68, TLR4, P-IkappaB and 
      TNF-alpha in liver were reduced. CONCLUSION: Jianpi Huoxue Decoction can inhibit 
      Kupffer cell signal pathway activation in rats with liver injury induced by 
      Lieber-Decarli liquid diet and LPS.
FAU - Peng, Jing-hua
AU  - Peng JH
AD  - Institute of Liver Diseases, Shuguang Hospital, Shanghai University of 
      Traditional Chinese Medicine, Shanghai 201203, China.
FAU - Fang, Zhi-hong
AU  - Fang ZH
FAU - Cui, Jian-wei
AU  - Cui JW
FAU - Feng, Qin
AU  - Feng Q
FAU - Xu, Li-li
AU  - Xu LL
FAU - Gu, Hong-tu
AU  - Gu HT
FAU - Hu, Yi-yang
AU  - Hu YY
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhong Xi Yi Jie He Xue Bao
JT  - Zhong xi yi jie he xue bao = Journal of Chinese integrative medicine
JID - 101199657
RN  - 0 (Antigens, CD)
RN  - 0 (Antigens, Differentiation, Myelomonocytic)
RN  - 0 (CD68 antigen, human)
RN  - 0 (Drugs, Chinese Herbal)
RN  - 0 (Jianpi Huoxue decoction)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Toll-Like Receptor 4)
RN  - 0 (Triglycerides)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - EC 2.6.1.2 (Alanine Transaminase)
SB  - IM
MH  - Alanine Transaminase/blood
MH  - Animals
MH  - Antigens, CD/metabolism
MH  - Antigens, Differentiation, Myelomonocytic/metabolism
MH  - Blotting, Western
MH  - Diet
MH  - Drugs, Chinese Herbal/pharmacology/*therapeutic use
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Kupffer Cells/*drug effects/metabolism/pathology
MH  - Lipopolysaccharides
MH  - Liver Diseases, Alcoholic/*drug therapy/etiology/pathology
MH  - Male
MH  - *Phytotherapy
MH  - Random Allocation
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Signal Transduction/drug effects
MH  - Toll-Like Receptor 4/metabolism
MH  - Triglycerides/metabolism
MH  - Tumor Necrosis Factor-alpha/blood/metabolism
EDAT- 2007/05/15 09:00
MHDA- 2008/04/15 09:00
CRDT- 2007/05/15 09:00
PHST- 2007/05/15 09:00 [pubmed]
PHST- 2008/04/15 09:00 [medline]
PHST- 2007/05/15 09:00 [entrez]
AID - 167219772007030302 [pii]
AID - 10.3736/jcim20070314 [doi]
PST - ppublish
SO  - Zhong Xi Yi Jie He Xue Bao. 2007 May;5(3):302-6. doi: 10.3736/jcim20070314.