PMID- 17499648
OWN - NLM
STAT- MEDLINE
DCOM- 20070720
LR  - 20220317
IS  - 0361-9230 (Print)
IS  - 0361-9230 (Linking)
VI  - 73
IP  - 1-3
DP  - 2007 Jun 15
TI  - Measurements of brain-derived neurotrophic factor: methodological aspects and 
      demographical data.
PG  - 143-9
AB  - Although numerous studies have dealt with changes in blood brain-derived 
      neurotrophic factor (BDNF), methodological issues about BDNF measurements have 
      only been incompletely resolved. We validated BDNF ELISA with respect to 
      accuracy, reproducibility and the effect of storage and repeated freezing cycles 
      on BDNF concentrations. Additionally, the effect of demographic characteristics 
      in healthy subjects on BDNF was verified. Whole blood and serum was collected 
      from 206 healthy subjects and a subgroup was genotyped for BDNF Val66Met 
      polymorphism. The effect of age, gender, BDNF genotype and thrombocyte count on 
      whole blood BDNF was assessed. The BDNF ELISA measurement was accurate, 
      91.6+/-3.0%, and showed high reproducibility, whereas inter-assay and 
      intra-subject variations were modest, 8.4+/-5.2% and 17.5+/-14.1%, respectively. 
      Storage of whole blood samples at 4 degrees C significantly decreased BDNF 
      concentration, while repeated freezing cycles and storage at -20 degrees C was 
      without any effect. Storage at -20 degrees C of serum, but not whole blood, was 
      associated with a significant decrease in BDNF concentration. Women had 
      significantly higher whole blood BDNF concentrations than men (18.6+/-1.3 ng/ml 
      versus 16.5+/-1.4 ng/ml), and showed a right-skewed BDNF concentration 
      distribution. No association between whole blood BDNF concentrations and 
      thrombocyte count, age, or BDNF genotype was found. In conclusion, the BDNF ELISA 
      assay determines whole blood BDNF accurately and with high reproducibility. 
      Female gender is associated with higher whole blood BDNF concentrations whereas 
      age, thrombocyte count and BDNF Val66Met polymorphism were un-associated.
FAU - Trajkovska, Viktorija
AU  - Trajkovska V
AD  - Neurobiology Research Unit and Center for Integrated Molecular Brain Imaging, 
      Rigshospitalet, Copenhagen University Hospital, Section 9201, Blegdamsvej 9, 
      DK-2100 Copenhagen, Denmark. viktorija.trajkovska@nru.dk
FAU - Marcussen, Anders B
AU  - Marcussen AB
FAU - Vinberg, Maj
AU  - Vinberg M
FAU - Hartvig, Per
AU  - Hartvig P
FAU - Aznar, Susana
AU  - Aznar S
FAU - Knudsen, Gitte M
AU  - Knudsen GM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20070409
PL  - United States
TA  - Brain Res Bull
JT  - Brain research bulletin
JID - 7605818
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aging/blood
MH  - Brain-Derived Neurotrophic Factor/*blood/genetics
MH  - DNA/genetics
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Female
MH  - Freezing
MH  - Genotype
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - Platelet Count
MH  - Polymorphism, Genetic/genetics
MH  - Reference Standards
MH  - Reproducibility of Results
MH  - Sex Characteristics
MH  - Specimen Handling
EDAT- 2007/05/15 09:00
MHDA- 2007/07/21 09:00
CRDT- 2007/05/15 09:00
PHST- 2007/01/03 00:00 [received]
PHST- 2007/03/15 00:00 [revised]
PHST- 2007/03/15 00:00 [accepted]
PHST- 2007/05/15 09:00 [pubmed]
PHST- 2007/07/21 09:00 [medline]
PHST- 2007/05/15 09:00 [entrez]
AID - S0361-9230(07)00104-9 [pii]
AID - 10.1016/j.brainresbull.2007.03.009 [doi]
PST - ppublish
SO  - Brain Res Bull. 2007 Jun 15;73(1-3):143-9. doi: 
      10.1016/j.brainresbull.2007.03.009. Epub 2007 Apr 9.