PMID- 17500240
OWN - NLM
STAT- MEDLINE
DCOM- 20070608
LR  - 20161109
IS  - 0507-4088 (Print)
IS  - 0507-4088 (Linking)
VI  - 52
IP  - 2
DP  - 2007 Mar-Apr
TI  - [Enzyme-linked immunosorbent assay for detection of antibodies to porcine 
      reproductive and respiratory syndrome virus, by using recombinant nucleocapsid 
      protein N].
PG  - 45-9
AB  - Recombinant nucleocapsid (rN) protein N of porcine reproductive and respiratory 
      syndrome virus (PRRSV) was prepared, by using the E. coli expressiom system. 
      Insertion of a polyhistidine marker into the structure of the protein allowed the 
      latter to be purified by metal-chelate affinity chromatography. The purity of 
      protein was confirmed by PAAG electrophoresis and its immunospecificity was 
      verified by immunoblotting using rN-specific monoclonal antibodies. The protein 
      was used as an antigen to develop indirect ELISA of PRRSV antibodies. ELISA was 
      shown to be highly sensitive and specific.
FAU - Bogdanova, V S
AU  - Bogdanova VS
FAU - Tsibezov, V V
AU  - Tsibezov VV
FAU - Grabovetskii, V V
AU  - Grabovetskii VV
FAU - Eliseeva, O V
AU  - Eliseeva OV
FAU - Grebennikova, T V
AU  - Grebennikova TV
FAU - Verkhovskii, O A
AU  - Verkhovskii OA
FAU - Zabereshchnyi, A D
AU  - Zabereshchnyi AD
FAU - Aliper, T I
AU  - Aliper TI
LA  - rus
PT  - English Abstract
PT  - Journal Article
PL  - Russia (Federation)
TA  - Vopr Virusol
JT  - Voprosy virusologii
JID - 0417337
RN  - 0 (Antibodies, Viral)
RN  - 0 (Nucleocapsid Proteins)
RN  - 0 (Reagent Kits, Diagnostic)
RN  - 0 (Recombinant Proteins)
SB  - IM
MH  - Animals
MH  - Antibodies, Viral/blood
MH  - Antibody Specificity
MH  - Enzyme-Linked Immunosorbent Assay/methods
MH  - Nucleocapsid Proteins/immunology
MH  - Porcine Reproductive and Respiratory Syndrome/blood/*diagnosis
MH  - Porcine respiratory and reproductive syndrome virus/*immunology
MH  - Reagent Kits, Diagnostic
MH  - Recombinant Proteins/immunology
MH  - Sensitivity and Specificity
MH  - Serologic Tests
MH  - Swine
EDAT- 2007/05/16 09:00
MHDA- 2007/06/09 09:00
CRDT- 2007/05/16 09:00
PHST- 2007/05/16 09:00 [pubmed]
PHST- 2007/06/09 09:00 [medline]
PHST- 2007/05/16 09:00 [entrez]
PST - ppublish
SO  - Vopr Virusol. 2007 Mar-Apr;52(2):45-9.