PMID- 17502662
OWN - NLM
STAT- MEDLINE
DCOM- 20071213
LR  - 20230507
IS  - 0022-1007 (Print)
IS  - 1540-9538 (Electronic)
IS  - 0022-1007 (Linking)
VI  - 204
IP  - 6
DP  - 2007 Jun 11
TI  - Type I interferons produced by hematopoietic cells protect mice against lethal 
      infection by mammalian reovirus.
PG  - 1349-58
AB  - We defined the function of type I interferons (IFNs) in defense against reovirus 
      strain type 1 Lang (T1L), which is a double-stranded RNA virus that infects 
      Peyer's patches (PPs) after peroral inoculation of mice. T1L induced expression 
      of mRNA for IFN-alpha, IFN-beta, and Mx-1 in PPs and caused localized intestinal 
      infection that was cleared in 10 d. In contrast, T1L produced fatal systemic 
      infection in IFNalphaR1 knockout (KO) mice with extensive cell loss in lymphoid 
      tissues and necrosis of the intestinal mucosa. Studies of bone-marrow chimeric 
      mice indicated an essential role for hematopoietic cells in IFN-dependent viral 
      clearance. Dendritic cells (DCs), including conventional DCs (cDCs), were the 
      major source of type I IFNs in PPs of reovirus-infected mice, whereas all cell 
      types expressed the antiviral protein Mx-1. Neither NK cells nor signaling via 
      Toll-like receptor 3 or MyD88 were essential for viral clearance. These data 
      demonstrate a requirement for type I IFNs in the control of an intestinal viral 
      infection and indicate that cDCs are a significant source of type I IFN 
      production in vivo. Therefore, innate immunity in PPs is an essential component 
      of host defense that limits systemic spread of pathogens that infect the 
      intestinal mucosa.
FAU - Johansson, Cecilia
AU  - Johansson C
AD  - Laboratory of Molecular Immunology, National Institute of Allergy and Infectious 
      Diseases, National Institutes of Health, Bethesda, MD 20892, USA.
FAU - Wetzel, J Denise
AU  - Wetzel JD
FAU - He, Jianping
AU  - He J
FAU - Mikacenic, Carmen
AU  - Mikacenic C
FAU - Dermody, Terence S
AU  - Dermody TS
FAU - Kelsall, Brian L
AU  - Kelsall BL
LA  - eng
GR  - P30 DK020593/DK/NIDDK NIH HHS/United States
GR  - P30 CA068485/CA/NCI NIH HHS/United States
GR  - AI50080/AI/NIAID NIH HHS/United States
GR  - DK20593/DK/NIDDK NIH HHS/United States
GR  - CA68485/CA/NCI NIH HHS/United States
GR  - R01 AI050080/AI/NIAID NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20070514
PL  - United States
TA  - J Exp Med
JT  - The Journal of experimental medicine
JID - 2985109R
RN  - 0 (Interferon Type I)
SB  - IM
MH  - Animals
MH  - Bone Marrow Cells/*immunology
MH  - Dendritic Cells/*immunology
MH  - Immunohistochemistry
MH  - Interferon Type I/genetics/*immunology/metabolism
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mice, Inbred C57BL
MH  - Mice, Knockout
MH  - Orthoreovirus, Mammalian/*immunology
MH  - Peyer's Patches/*immunology/virology
MH  - Reoviridae Infections/immunology/*prevention & control
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Survival Analysis
PMC - PMC2118611
EDAT- 2007/05/16 09:00
MHDA- 2007/12/14 09:00
PMCR- 2007/12/11
CRDT- 2007/05/16 09:00
PHST- 2007/05/16 09:00 [pubmed]
PHST- 2007/12/14 09:00 [medline]
PHST- 2007/05/16 09:00 [entrez]
PHST- 2007/12/11 00:00 [pmc-release]
AID - jem.20061587 [pii]
AID - 20061587 [pii]
AID - 10.1084/jem.20061587 [doi]
PST - ppublish
SO  - J Exp Med. 2007 Jun 11;204(6):1349-58. doi: 10.1084/jem.20061587. Epub 2007 May 
      14.