PMID- 17503441
OWN - NLM
STAT- MEDLINE
DCOM- 20080624
LR  - 20161124
IS  - 0952-3480 (Print)
IS  - 0952-3480 (Linking)
VI  - 21
IP  - 2
DP  - 2008 Feb
TI  - Magnetically enriched bone marrow-derived macrophages loaded in vitro with iron 
      oxide can migrate to inflammation sites in mice.
PG  - 120-8
AB  - In vitro labelling of cells permits incorporation of large amounts of iron oxide 
      and consequently high detection sensitivity, but it remains controversial whether 
      labelled cells would respond normally to stimuli. This question was addressed by 
      differentiating bone marrow-derived macrophages (BMDMs) in vitro, labelling cells 
      with high concentrations of Endorem in vitro, and eliminating unlabelled cells by 
      magnetic enrichment. To explore their acute inflammatory response, enriched cells 
      were injected into mice with carrageenan-induced inflammation, the 'air pouch 
      model'. Cells recovered from the inflammation site 16 h after intravenous BMDM 
      injection into the tail vein were analysed by in vitro MRI and fluorescent 
      microscopy. With both assays, Endorem-labelled cells were detectable. This 
      indicates that BMDMs, loaded with high concentrations of iron oxide in vitro, can 
      still respond to chemokine gradients and infiltrate inflamed tissue in mice. 
      Furthermore, by using genetically modified mice as BMDM donors, it should be 
      possible to study the role of individual genes in macrophage recruitment.
FAU - Hamm, Jorg
AU  - Hamm J
AD  - Department of Genetics, Biology and Biochemistry and CERMS, University of Turin, 
      Turin, Italy. jorg_hamm@yahoo.com
FAU - Pulito, Roberta
AU  - Pulito R
FAU - Benedetto, Sabrina
AU  - Benedetto S
FAU - Barberis, Laura
AU  - Barberis L
FAU - Hirsch, Emilio
AU  - Hirsch E
FAU - Poli, Valeria
AU  - Poli V
FAU - Silengo, Lorenzo
AU  - Silengo L
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - NMR Biomed
JT  - NMR in biomedicine
JID - 8915233
RN  - 0 (Dextrans)
RN  - 0 (Ferric Compounds)
RN  - 0 (Magnetite Nanoparticles)
RN  - 0 (Oxides)
RN  - 0 (Suspensions)
RN  - 1K09F3G675 (ferric oxide)
RN  - 9000-07-1 (Carrageenan)
RN  - E1UOL152H7 (Iron)
RN  - G6N3J05W84 (ferumoxides)
RN  - XM0M87F357 (Ferrosoferric Oxide)
SB  - IM
MH  - Animals
MH  - Bone Marrow Cells/*cytology/*metabolism
MH  - Carrageenan/immunology
MH  - Cell Differentiation
MH  - Dextrans
MH  - Disease Models, Animal
MH  - Ferric Compounds/*adverse effects
MH  - Ferrosoferric Oxide
MH  - Foreign-Body Reaction/*chemically induced
MH  - Iron/adverse effects
MH  - Macrophage Activation/*drug effects
MH  - Macrophages/cytology/immunology/transplantation
MH  - Magnetic Resonance Imaging
MH  - Magnetite Nanoparticles
MH  - Male
MH  - Mice
MH  - Microscopy, Fluorescence
MH  - Oxides/adverse effects
MH  - Research Design
MH  - Suspensions
EDAT- 2007/05/16 09:00
MHDA- 2008/06/25 09:00
CRDT- 2007/05/16 09:00
PHST- 2007/05/16 09:00 [pubmed]
PHST- 2008/06/25 09:00 [medline]
PHST- 2007/05/16 09:00 [entrez]
AID - 10.1002/nbm.1168 [doi]
PST - ppublish
SO  - NMR Biomed. 2008 Feb;21(2):120-8. doi: 10.1002/nbm.1168.