PMID- 17504486
OWN - NLM
STAT- MEDLINE
DCOM- 20070724
LR  - 20070516
IS  - 1462-2912 (Print)
IS  - 1462-2912 (Linking)
VI  - 9
IP  - 6
DP  - 2007 Jun
TI  - Ecophysiology of Defluviicoccus-related tetrad-forming organisms in an 
      anaerobic-aerobic activated sludge process.
PG  - 1485-96
AB  - A group of uncultured tetrad-forming organisms (TFOs) was enriched in an 
      acetate-fed anaerobic-aerobic sequencing membrane bioreactor showing deteriorated 
      enhanced biological phosphorus removal capacity. Based on 16S rRNA gene clone 
      library and fluorescence in situ hybridization (FISH) analyses, these TFOs were 
      identified as novel members of the Defluviicoccus cluster in the 
      Alphaproteobacteria, accounting for 90 +/- 5% of the EUBmix FISH-detectable 
      bacterial cell area in the reactor biomass. Microautoradiography in combination 
      with FISH and polyhydroxyalkanoate (PHA) staining revealed that these 
      Defluviicoccus-related TFOs could take up and transform acetate, lactate, 
      propionate and pyruvate, but not aspartic acid and glucose, into PHA under 
      anaerobic conditions. In contrast, under continuous anaerobic-aerobic 
      cultivation, Defluviicoccus vanus, the only cultured strain from the cluster, was 
      able to take up glucose with concurrent glycogen consumption and PHA production 
      under anaerobic conditions. Under subsequent aerobic conditions, the accumulated 
      PHA was utilized and the biomass glycogen levels were restored. These findings 
      not only re-confirmed these Defluviicoccus-related TFOs as glycogen-accumulating 
      organisms, but also revealed unexpected levels of physiological, phylogenetic and 
      morphological diversity among members of the Defluviicoccus cluster.
FAU - Wong, Man-Tak
AU  - Wong MT
AD  - Division of Environmental Science and Engineering, National University of 
      Singapore, Singapore 117576.
FAU - Liu, Wen-Tso
AU  - Liu WT
LA  - eng
SI  - GENBANK/DQ250532
SI  - GENBANK/DQ250533
SI  - GENBANK/DQ250534
SI  - GENBANK/DQ250535
SI  - GENBANK/DQ250536
SI  - GENBANK/DQ250537
SI  - GENBANK/DQ250538
SI  - GENBANK/DQ250539
SI  - GENBANK/DQ250540
PT  - Journal Article
PL  - England
TA  - Environ Microbiol
JT  - Environmental microbiology
JID - 100883692
RN  - 0 (RNA, Bacterial)
RN  - 0 (RNA, Ribosomal, 16S)
RN  - 0 (Sewage)
RN  - 9005-79-2 (Glycogen)
SB  - IM
MH  - Alphaproteobacteria/*classification/genetics/*metabolism
MH  - Anaerobiosis/*physiology
MH  - Bacteria, Anaerobic/*physiology
MH  - Biodegradation, Environmental
MH  - Glycogen/*metabolism
MH  - Molecular Sequence Data
MH  - RNA, Bacterial/genetics
MH  - RNA, Ribosomal, 16S/genetics
MH  - Sequence Analysis, DNA
MH  - Sewage/*microbiology
MH  - Waste Disposal, Fluid
EDAT- 2007/05/17 09:00
MHDA- 2007/07/25 09:00
CRDT- 2007/05/17 09:00
PHST- 2007/05/17 09:00 [pubmed]
PHST- 2007/07/25 09:00 [medline]
PHST- 2007/05/17 09:00 [entrez]
AID - EMI1267 [pii]
AID - 10.1111/j.1462-2920.2007.01267.x [doi]
PST - ppublish
SO  - Environ Microbiol. 2007 Jun;9(6):1485-96. doi: 10.1111/j.1462-2920.2007.01267.x.