PMID- 17505982
OWN - NLM
STAT- MEDLINE
DCOM- 20070712
LR  - 20181113
IS  - 1355-0284 (Print)
IS  - 1355-0284 (Linking)
VI  - 13
IP  - 2
DP  - 2007 Apr
TI  - A protein encoded by the bovine herpesvirus 1 open reading frame E gene induces 
      neurite-like morphological changes in mouse neuroblastoma cells and is expressed 
      in trigeminal ganglionic neurons.
PG  - 139-49
AB  - Bovine herpes virus 1 (BHV-1), like other alpha-herpesvirinae subfamily members, 
      establishes latency in sensory neurons. Periodically BHV-1 reactivates from 
      latency, resulting in virus shedding and spread to uninfected cattle. Although 
      reactivation from latency does not usually lead to recurrent disease, the 
      latency-reactivation cycle is crucial for virus transmission. The latency-related 
      (LR) RNA is abundantly expressed during latency, and expression of a LR encoded 
      protein is necessary for dexamethasone-induced reactivation from latency in 
      cattle. Within LR promoter sequences, a small open reading frame (ORF) was 
      identified (ORF-E) that is antisense to the LR-RNA, and downstream of the bICP0 
      gene. ORF-E transcription is consistently detected in trigeminal ganglia (TG) of 
      latently infected calves, suggesting ORF-E expression plays a role in the 
      latency-reactivation cycle. Polyclonal antiserum directed against an ORF-E 
      peptide or the entire ORF-E protein specifically recognizes the nucleus of 
      sensory neurons in TG of latently infected calves. The ORF-E peptide-specific 
      antiserum also recognizes a protein when mouse neuroblastoma cells (neuro-2A) are 
      transfected with an ORF-E expression construct. In contrast to the growth 
      inhibiting properties of the LR gene, stably transfected ORF-E-expressing cells 
      were obtained. Neuro-2A cells stably transfected with a plasmid expressing ORF-E 
      induced morphological changes that resembled neurite-like projections. In 
      contrast, neurite-like projections were not observed following transfection of 
      neuro-2A cells with an empty vector. These studies suggest that a protein encoded 
      by ORF-E has the potential to alter the physiology or metabolism of neuronal cell 
      types, which may be important for long-term latency.
FAU - Perez, Sandra
AU  - Perez S
AD  - Department of Veterinary and Biomedical Sciences, University of Nebraska, 
      Lincoln, Nebraska 68583-0905, USA.
FAU - Meyer, Florencia
AU  - Meyer F
FAU - Henderson, Gail
AU  - Henderson G
FAU - Jiang, Yunquan
AU  - Jiang Y
FAU - Sherman, Simon
AU  - Sherman S
FAU - Doster, Alan
AU  - Doster A
FAU - Inman, Melissa
AU  - Inman M
FAU - Jones, Clinton
AU  - Jones C
LA  - eng
GR  - P20RR15635/RR/NCRR NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - J Neurovirol
JT  - Journal of neurovirology
JID - 9508123
RN  - 0 (Viral Fusion Proteins)
RN  - 0 (Viral Proteins)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Cattle
MH  - Cattle Diseases/pathology/*virology
MH  - Cell Line
MH  - Herpesviridae Infections/pathology/*veterinary/virology
MH  - *Herpesvirus 1, Bovine/chemistry/physiology
MH  - Immunohistochemistry
MH  - Mice
MH  - Molecular Sequence Data
MH  - Neurites/*pathology
MH  - Open Reading Frames/physiology
MH  - Transfection
MH  - Trigeminal Ganglion/*metabolism
MH  - Viral Fusion Proteins/*physiology
MH  - Viral Proteins/*physiology
MH  - Virus Latency
EDAT- 2007/05/17 09:00
MHDA- 2007/07/13 09:00
CRDT- 2007/05/17 09:00
PHST- 2007/05/17 09:00 [pubmed]
PHST- 2007/07/13 09:00 [medline]
PHST- 2007/05/17 09:00 [entrez]
AID - 778724457 [pii]
AID - 10.1080/13550280701191459 [doi]
PST - ppublish
SO  - J Neurovirol. 2007 Apr;13(2):139-49. doi: 10.1080/13550280701191459.