PMID- 17506853
OWN - NLM
STAT- MEDLINE
DCOM- 20071109
LR  - 20181113
IS  - 0019-2805 (Print)
IS  - 1365-2567 (Electronic)
IS  - 0019-2805 (Linking)
VI  - 122
IP  - 2
DP  - 2007 Oct
TI  - Chronic intranasal administration of mould spores or extracts to unsensitized 
      mice leads to lung allergic inflammation, hyper-reactivity and remodelling.
PG  - 268-78
AB  - Allergic asthma is a serious multifaceted disease characterized by 
      eosinophil-rich airway inflammation, airway hyperreactivity and airway wall 
      modifications known as remodelling. We previously demonstrated that the spores of 
      two allergenic moulds, Alternaria alternata and Cladosporium herbarum, were 
      potent inducers of immunoglobulin E (IgE) production. Moreover, mice sensitized 
      by two intraperitoneal injections before intranasal challenge with A. alternata 
      or C. herbarum spores developed an allergic lung inflammation and 
      hyperreactivity. Here we report on the effect of chronic intranasal 
      administration of C. herbarum spores or A. alternata extracts to unsensitized 
      BALB/c mice. Our results demonstrate that this chronic treatment led to an 
      increase of total serum IgE and the appearance of specific IgE and IgG1. Total 
      cell number in bronchoalveolar lavage fluid from treated mice was highly 
      increased compared to phosphate-buffered-saline-treated mice because of the 
      accumulation of macrophages, neutrophils, lymphocytes and eosinophils. Airway 
      hyperreactivity appeared after 3 weeks (extract) and 7 weeks (spores) and was 
      maintained during the whole treatment. Increased interleukin-13 mRNA expression 
      in the lungs and T helper type 2 cytokines (interleukin-4, -5, -6 and -13) and 
      transforming growth factor-beta secretion in bronchoalveolar lavage fluid were 
      also observed. Lung hydroxyproline and fibronectin contents indicated increased 
      fibrosis in mice treated with mould allergen. These observations were confirmed 
      by histological analysis demonstrating airway wall remodelling and strong mucus 
      production. These observations show that this model, using chronic intranasal 
      administration of relevant particulate allergens, is an interesting tool for the 
      study of mechanisms leading to allergic pulmonary diseases and lung remodelling.
FAU - Denis, Olivier
AU  - Denis O
AD  - Allergology Unit, WIV-Pasteur Institute of Brussels, Brussels, Belgium. 
      odenis@pasteur.be
FAU - van den Brule, Sybille
AU  - van den Brule S
FAU - Heymans, Julie
AU  - Heymans J
FAU - Havaux, Xavier
AU  - Havaux X
FAU - Rochard, Christelle
AU  - Rochard C
FAU - Huaux, Francois
AU  - Huaux F
FAU - Huygen, Kris
AU  - Huygen K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20070516
PL  - England
TA  - Immunology
JT  - Immunology
JID - 0374672
RN  - 0 (Antibodies, Fungal)
RN  - 0 (Cytokines)
RN  - 0 (Immunoglobulin G)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Administration, Intranasal
MH  - Alternaria/immunology
MH  - Animals
MH  - Antibodies, Fungal/biosynthesis
MH  - Asthma/*immunology/pathology
MH  - Bronchial Hyperreactivity/*immunology/pathology
MH  - Bronchoalveolar Lavage Fluid/cytology
MH  - Cladosporium/immunology
MH  - Cytokines/biosynthesis
MH  - Disease Models, Animal
MH  - Female
MH  - Immunoglobulin E/biosynthesis
MH  - Immunoglobulin G/biosynthesis
MH  - Lung/immunology/pathology
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Spores, Fungal/*immunology
MH  - Th2 Cells/immunology
PMC - PMC2265999
EDAT- 2007/05/18 09:00
MHDA- 2007/11/10 09:00
PMCR- 2008/10/01
CRDT- 2007/05/18 09:00
PHST- 2007/05/18 09:00 [pubmed]
PHST- 2007/11/10 09:00 [medline]
PHST- 2007/05/18 09:00 [entrez]
PHST- 2008/10/01 00:00 [pmc-release]
AID - IMM2636 [pii]
AID - 10.1111/j.1365-2567.2007.02636.x [doi]
PST - ppublish
SO  - Immunology. 2007 Oct;122(2):268-78. doi: 10.1111/j.1365-2567.2007.02636.x. Epub 
      2007 May 16.