PMID- 17507470
OWN - NLM
STAT- MEDLINE
DCOM- 20070926
LR  - 20240316
IS  - 0022-538X (Print)
IS  - 1098-5514 (Electronic)
IS  - 0022-538X (Linking)
VI  - 81
IP  - 15
DP  - 2007 Aug
TI  - Establishment of a poliovirus oral infection system in human poliovirus 
      receptor-expressing transgenic mice that are deficient in alpha/beta interferon 
      receptor.
PG  - 7902-12
AB  - Poliovirus (PV) is easily transferred to humans orally; however, no rodent model 
      for oral infections has been developed because of the alimentary tract's low 
      sensitivity to the virus. Here we showed that PV is inactivated by the low pH of 
      the gastric contents in mice. The addition of 3% NaHCO3 to the viral inoculum 
      increased the titer of virus reaching the small intestine through the stomach 
      after intragastric inoculation of PV. Transgenic mice (Tg) carrying the human PV 
      receptor (hPVR/CD155) gene and lacking the alpha/beta interferon receptor (IFNAR) 
      gene (hPVR-Tg/IfnarKO) were sensitive to the oral administration of PV with 3% 
      NaHCO3, whereas hPVR-Tg expressing IFNAR were much less sensitive. The virus was 
      detected in the epithelia of the small intestine and proliferated in the 
      alimentary tract of hPVR-Tg/IfnarKO. By the ninth day after the administration of 
      a virulent PV, the mice had died. These results suggest that IFNAR plays an 
      important role in determining permissivity in the alimentary tract as well as the 
      generation of virus-specific immune responses to PV via the oral route. Thus, 
      hPVR-Tg/IfnarKO are considered to be the first oral infection model for PV, 
      although levels of anti-PV antibodies were not elevated dramatically in serum and 
      intestinal secretions of surviving mice when hPVR-Tg/IfnarKO were administered an 
      attenuated PV.
FAU - Ohka, Seii
AU  - Ohka S
AD  - Department of Microbiology, Graduate School of Medicine, The University of Tokyo, 
      7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan. seii@m.u-tokyo.ac.jp
FAU - Igarashi, Hiroko
AU  - Igarashi H
FAU - Nagata, Noriyo
AU  - Nagata N
FAU - Sakai, Mai
AU  - Sakai M
FAU - Koike, Satoshi
AU  - Koike S
FAU - Nochi, Tomonori
AU  - Nochi T
FAU - Kiyono, Hiroshi
AU  - Kiyono H
FAU - Nomoto, Akio
AU  - Nomoto A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20070516
PL  - United States
TA  - J Virol
JT  - Journal of virology
JID - 0113724
RN  - 0 (Antibodies)
RN  - 0 (Membrane Proteins)
RN  - 0 (Receptors, Virus)
RN  - 0 (poliovirus receptor)
RN  - 156986-95-7 (Receptor, Interferon alpha-beta)
SB  - IM
MH  - Administration, Oral
MH  - Animals
MH  - Antibodies/metabolism
MH  - *Disease Models, Animal
MH  - Humans
MH  - Intestines/cytology/virology
MH  - Membrane Proteins/genetics/*metabolism
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Knockout
MH  - *Mice, Transgenic
MH  - *Poliovirus/metabolism/pathogenicity
MH  - Receptor, Interferon alpha-beta/genetics/*metabolism
MH  - Receptors, Virus/genetics/*metabolism
MH  - Signal Transduction/physiology
MH  - Virus Replication
PMC - PMC1951287
EDAT- 2007/05/18 09:00
MHDA- 2007/09/27 09:00
PMCR- 2007/12/01
CRDT- 2007/05/18 09:00
PHST- 2007/05/18 09:00 [pubmed]
PHST- 2007/09/27 09:00 [medline]
PHST- 2007/05/18 09:00 [entrez]
PHST- 2007/12/01 00:00 [pmc-release]
AID - JVI.02675-06 [pii]
AID - 2675-06 [pii]
AID - 10.1128/JVI.02675-06 [doi]
PST - ppublish
SO  - J Virol. 2007 Aug;81(15):7902-12. doi: 10.1128/JVI.02675-06. Epub 2007 May 16.