PMID- 17509021
OWN - NLM
STAT- MEDLINE
DCOM- 20070613
LR  - 20171116
IS  - 1350-1925 (Print)
IS  - 1350-1925 (Linking)
VI  - 19
IP  - 5
DP  - 2007 May
TI  - Stimulation of neuronal receptors, neuropeptides and cytokines during 
      experimental oil of mustard colitis.
PG  - 390-400
AB  - Oil of mustard (OM), administered intracolonically, produces severe colitis in 
      mice that is maximized within 3 days. The purpose of this study was to 
      characterize the cytokine response, and to establish expression patterns of 
      enteric neuronal mediators and neuronal receptors affected during active colitis. 
      We measured the changes in the mRNA levels for neuronal receptors and mediators 
      by real-time PCR, and cytokine and chemokine protein levels in the affected 
      tissue. Significant increases in neuronal receptors, such as transient receptor 
      potential A1 (TRPA1), cannabinoid type 1 receptor, neurokinin 1 receptor (NK1R) 
      and delta-opioid receptor; prokineticin-1 receptor; and soluble mediators, such 
      as prodynorphin, proenkephalin1, NK1, prokineticin-1 and secretory leukocyte 
      protease inhibitor, occurred. Significant increases in cytokines, such as 
      interleukin (IL)-1beta, IL-6 and granulocyte macrophage colony stimulating factor 
      (GM-CSF), and in chemokines, such as macrophage chemotactic protein 1 (MCP-1), 
      macrophage inflammatory protein 1 (MIP-1alpha) and Kupffer cell derived chemokine 
      (KC), were detected, with no changes in T-cell-derived cytokines. Furthermore, 
      immunodeficient C57Bl/6 RAG2(-/-) mice exhibited OM colitis of equal severity as 
      seen in wt C57Bl/6 and CD-1 mice. The results demonstrate rapidly increased 
      levels of mRNA for neuronal receptors and soluble mediators associated with pain 
      and inflammation, and increases in cytokines associated with macrophage and 
      neutrophil activation and recruitment. Collectively, the data support a 
      neurogenic component in OM colitis coupled with a myeloid cell-related, T- and 
      B-cell-independent inflammatory component.
FAU - Kimball, E S
AU  - Kimball ES
AD  - Enterology Research Team, Johnson and Johnson Pharmaceutical Research and 
      Development, Spring House, PA 19477, USA. kimballa@verizon.net
FAU - Prouty, S P
AU  - Prouty SP
FAU - Pavlick, K P
AU  - Pavlick KP
FAU - Wallace, N H
AU  - Wallace NH
FAU - Schneider, C R
AU  - Schneider CR
FAU - Hornby, P J
AU  - Hornby PJ
LA  - eng
PT  - Journal Article
PL  - England
TA  - Neurogastroenterol Motil
JT  - Neurogastroenterology and motility
JID - 9432572
RN  - 0 (Cytokines)
RN  - 0 (Neuropeptides)
RN  - 0 (Plant Oils)
RN  - 0 (TRPA1 Cation Channel)
RN  - 0 (TRPV Cation Channels)
RN  - 0 (TRPV1 protein, mouse)
RN  - 0 (Transient Receptor Potential Channels)
RN  - 0 (Trpa1 protein, mouse)
RN  - TYY1MA9BSY (mustard oil)
SB  - IM
MH  - Animals
MH  - Colitis/*chemically induced/pathology
MH  - Colon/metabolism/pathology
MH  - Cytokines/genetics/*metabolism
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Knockout/genetics/metabolism
MH  - Mustard Plant/*toxicity
MH  - Neuropeptides/genetics/*metabolism
MH  - Plant Oils/administration & dosage/*toxicity
MH  - Sensory Receptor Cells/*metabolism
MH  - TRPA1 Cation Channel
MH  - TRPV Cation Channels/genetics/metabolism
MH  - Transient Receptor Potential Channels/genetics/metabolism
EDAT- 2007/05/19 09:00
MHDA- 2007/06/15 09:00
CRDT- 2007/05/19 09:00
PHST- 2007/05/19 09:00 [pubmed]
PHST- 2007/06/15 09:00 [medline]
PHST- 2007/05/19 09:00 [entrez]
AID - NMO939 [pii]
AID - 10.1111/j.1365-2982.2007.00939.x [doi]
PST - ppublish
SO  - Neurogastroenterol Motil. 2007 May;19(5):390-400. doi: 
      10.1111/j.1365-2982.2007.00939.x.