PMID- 17511422
OWN - NLM
STAT- MEDLINE
DCOM- 20071016
LR  - 20181201
IS  - 0003-2700 (Print)
IS  - 0003-2700 (Linking)
VI  - 79
IP  - 12
DP  - 2007 Jun 15
TI  - Development of a monoclonal antibody-based broad-specificity ELISA for 
      fluoroquinolone antibiotics in foods and molecular modeling studies of 
      cross-reactive compounds.
PG  - 4471-83
AB  - A competitive indirect enzyme-linked immunosorbent assay (ciELISA) using 
      monoclonal antibodies (Mabs) having broad specificity for fluoroquinolone (FQ) 
      antibiotics is described. Four FQs, ciprofloxacin (CIP), enrofloxacin (ENR), 
      norfloxacin (NOR), and ofloxacin (OFL), were conjugated to bovine serum albumin 
      for immunogens and to ovalbumin for coating antigens. A Mab C4A9H1 raised against 
      the CIP hapten exhibited high cross-reactivity (35-100%) with 12 of 14 FQs and 
      detected these FQs in a ciELISA below their maximum residue levels (MRLs) with 
      good sensitivity at 50% binding inhibition (IC50). The quantitative 
      structure-activity relationship (QSAR) between Mab C4A9H1 and various FQs by 
      comparative molecular field analysis (CoMFA) showed a high predictive ability 
      with a cross-validation q2 value of 0.866. Using a simple purification process 
      and the broad-specificity ciELISA adapted for analysis of FQs in chicken muscle, 
      chicken liver, honey, shrimp, and whole egg samples demonstrated recoveries of 
      60-93% for CIP, ENR, NOR, OFL, flumequine, and danofloxacin.
FAU - Wang, Zhanhui
AU  - Wang Z
AD  - Department of Veterinary Pharmacology and Toxicology, College of Veterinary 
      Medicine, China Agricultural University, Beijing 100094, People's Republic of 
      China.
FAU - Zhu, Yan
AU  - Zhu Y
FAU - Ding, Shuangyang
AU  - Ding S
FAU - He, Fangyang
AU  - He F
FAU - Beier, Ross C
AU  - Beier RC
FAU - Li, Jiancheng
AU  - Li J
FAU - Jiang, Haiyang
AU  - Jiang H
FAU - Feng, Caiwei
AU  - Feng C
FAU - Wan, Yuping
AU  - Wan Y
FAU - Zhang, Suxia
AU  - Zhang S
FAU - Kai, Zhenpeng
AU  - Kai Z
FAU - Yang, Xinling
AU  - Yang X
FAU - Shen, Jianzhong
AU  - Shen J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20070519
PL  - United States
TA  - Anal Chem
JT  - Analytical chemistry
JID - 0370536
RN  - 0 (Anti-Bacterial Agents)
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Fluoroquinolones)
RN  - 3DX3XEK1BN (Enrofloxacin)
RN  - 5E8K9I0O4U (Ciprofloxacin)
RN  - A4P49JAZ9H (Ofloxacin)
RN  - N0F8P22L1P (Norfloxacin)
SB  - IM
MH  - Animals
MH  - Anti-Bacterial Agents/*analysis
MH  - Antibodies, Monoclonal/*immunology
MH  - Antibody Specificity
MH  - Ciprofloxacin/analysis
MH  - Enrofloxacin
MH  - Enzyme-Linked Immunosorbent Assay/*methods
MH  - Fluoroquinolones/*analysis
MH  - Food Contamination/*analysis
MH  - Inhibitory Concentration 50
MH  - Norfloxacin/analysis
MH  - Ofloxacin/analysis
MH  - Quantitative Structure-Activity Relationship
MH  - Sensitivity and Specificity
EDAT- 2007/05/22 09:00
MHDA- 2007/10/17 09:00
CRDT- 2007/05/22 09:00
PHST- 2007/05/22 09:00 [pubmed]
PHST- 2007/10/17 09:00 [medline]
PHST- 2007/05/22 09:00 [entrez]
AID - 10.1021/ac070064t [doi]
PST - ppublish
SO  - Anal Chem. 2007 Jun 15;79(12):4471-83. doi: 10.1021/ac070064t. Epub 2007 May 19.