PMID- 17512002
OWN - NLM
STAT- MEDLINE
DCOM- 20071206
LR  - 20070723
IS  - 0022-1910 (Print)
IS  - 0022-1910 (Linking)
VI  - 53
IP  - 7
DP  - 2007 Jul
TI  - Cloning and characterization of two Campoletis chlorideae ichnovirus vankyrin 
      genes expressed in parasitized host Helicoverpa armigera.
PG  - 699-707
AB  - Polydnaviruses, symbionts of parasitic ichneumonid (ichnoviruses, IVs) and 
      braconid (bracoviruses, BVs), are injected into hosts along with wasp eggs. 
      Within the host, PDV genes are expressed and their products function to alter 
      lepidopteran host physiology and enable endoparasitoid development. In the 
      present study, we describe two Campoletis chlorideae ichnovirus (CcIV) viral 
      ankyrin (vankyrin) genes and their transcription. The CcIV vankyrin genes possess 
      ankyrin repeat domains that resemble the inhibitory domains of the Drosophila 
      melanogaster NF-kappaB transcription factor inhibitor (IkappaB) cactus. The 
      expression of CcIV vankyrin genes could be detected in Helicoverpa armigera 
      during the whole course of parasitization with two expression peaks, 30 min 
      post-parasitization (p.p.) and 2 days p.p. Our data indicate that the CcIV 
      vankyrin genes are differentially expressed in the tissues of parasitized hosts 
      and both are mainly expressed in hemocytes. The temporal and spatial variation in 
      expression of the two CcIV vankyrin genes suggests that CcIV vankyrin genes could 
      be involved in early protection of parasitoid eggs from host cellular immune 
      response by suppressing NF-kappaB signaling cascades, thereby altering 
      development and immune responses of parasitized lepidopteran hosts.
FAU - Tian, Shen-Peng
AU  - Tian SP
AD  - State Key Laboratory of Integrated Management of Insects and Rodents, Institute 
      of Zoology, Chinese Academy of Sciences, Beijing 100080, China.
FAU - Zhang, Ji-Hong
AU  - Zhang JH
FAU - Wang, Chen-Zhu
AU  - Wang CZ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20070410
PL  - England
TA  - J Insect Physiol
JT  - Journal of insect physiology
JID - 2985080R
RN  - 0 (DNA, Viral)
RN  - 0 (Oligodeoxyribonucleotides)
RN  - 0 (RNA, Viral)
RN  - 0 (Viral Proteins)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Conserved Sequence
MH  - DNA, Viral/chemistry/genetics
MH  - Gene Expression Regulation, Viral
MH  - Molecular Sequence Data
MH  - Oligodeoxyribonucleotides/chemistry
MH  - Phylogeny
MH  - Polydnaviridae/classification/*genetics/pathogenicity
MH  - Polymerase Chain Reaction
MH  - RNA, Viral/genetics/isolation & purification
MH  - Sequence Alignment
MH  - Viral Proteins/chemistry/genetics
MH  - Wasps/*virology
EDAT- 2007/05/22 09:00
MHDA- 2007/12/07 09:00
CRDT- 2007/05/22 09:00
PHST- 2006/11/25 00:00 [received]
PHST- 2007/03/29 00:00 [revised]
PHST- 2007/03/29 00:00 [accepted]
PHST- 2007/05/22 09:00 [pubmed]
PHST- 2007/12/07 09:00 [medline]
PHST- 2007/05/22 09:00 [entrez]
AID - S0022-1910(07)00086-8 [pii]
AID - 10.1016/j.jinsphys.2007.03.015 [doi]
PST - ppublish
SO  - J Insect Physiol. 2007 Jul;53(7):699-707. doi: 10.1016/j.jinsphys.2007.03.015. 
      Epub 2007 Apr 10.