PMID- 17512285
OWN - NLM
STAT- MEDLINE
DCOM- 20070710
LR  - 20220224
IS  - 1879-1883 (Electronic)
IS  - 0002-9610 (Linking)
VI  - 193
IP  - 6
DP  - 2007 Jun
TI  - Lipopolysaccharide activation of pericyte's Toll-like receptor-4 regulates 
      co-culture permeability.
PG  - 730-5
AB  - BACKGROUND: Pericytes (PCs) have a synergistic relationship with endothelial 
      cells (MVEC) in regulating capillary permeability. PCs express Toll-like 
      receptor-4 (TLR-4). We hypothesize one mechanism of MVEC/PC co-culture 
      permeability is regulated through lipopolysaccharide (LPS) activation of pericyte 
      TLR-4. METHODS: Rat PCs were harvested and cultured. PCs were transfected with 
      siRNA targeted to TLR-4. Western blotting was used to confirm gene silencing of 
      TLR-4. A previously described co-culture permeability assay was performed after 
      LPS treatment. RESULTS: Western blot confirmed successful silencing of TLR-4 in 
      PCs, which was sustained for 7 days. A dose- and time-dependent effect of LPS on 
      albumin clearance was seen in MVEC/PC co-cultures. Co-cultures with TLR-4 
      silenced in PCs eliminated the LPS dose-dependent increase in albumin clearance. 
      CONCLUSIONS: TLR-4 regulates pericyte mediated capillary leak seen with LPS 
      exposure. Our TLR-4 silencing model can be used to further investigate TLR-4's 
      role in pericyte mediated capillary leak.
FAU - Edelman, David A
AU  - Edelman DA
AD  - Department of Surgery, Wayne State University, 3900 John R, Suite 400 POB, 
      Detroit, MI 48201, USA.
FAU - Jiang, Yang
AU  - Jiang Y
FAU - Tyburski, James G
AU  - Tyburski JG
FAU - Wilson, Robert F
AU  - Wilson RF
FAU - Steffes, Christopher P
AU  - Steffes CP
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Am J Surg
JT  - American journal of surgery
JID - 0370473
RN  - 0 (Albumins)
RN  - 0 (Polysaccharides)
RN  - 0 (RNA, Small Interfering)
RN  - 0 (Tlr4 protein, rat)
RN  - 0 (Toll-Like Receptor 4)
SB  - IM
MH  - Albumins/metabolism
MH  - Animals
MH  - Blotting, Western
MH  - Cell Membrane Permeability/drug effects/genetics
MH  - Cells, Cultured
MH  - Coculture Techniques
MH  - Endothelial Cells/cytology/drug effects/*metabolism
MH  - Endothelium, Vascular/*cytology/drug effects/metabolism
MH  - Gene Silencing/*drug effects
MH  - Lung/blood supply/cytology
MH  - Pericytes/cytology/drug effects/*metabolism
MH  - Polysaccharides/*pharmacology
MH  - RNA, Small Interfering/*genetics
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Toll-Like Receptor 4/drug effects/*genetics/metabolism
EDAT- 2007/05/22 09:00
MHDA- 2007/07/11 09:00
CRDT- 2007/05/22 09:00
PHST- 2006/07/20 00:00 [received]
PHST- 2006/08/24 00:00 [revised]
PHST- 2006/08/24 00:00 [accepted]
PHST- 2007/05/22 09:00 [pubmed]
PHST- 2007/07/11 09:00 [medline]
PHST- 2007/05/22 09:00 [entrez]
AID - S0002-9610(07)00202-4 [pii]
AID - 10.1016/j.amjsurg.2006.08.086 [doi]
PST - ppublish
SO  - Am J Surg. 2007 Jun;193(6):730-5. doi: 10.1016/j.amjsurg.2006.08.086.