PMID- 17513594
OWN - NLM
STAT- MEDLINE
DCOM- 20071019
LR  - 20181113
IS  - 0099-2240 (Print)
IS  - 1098-5336 (Electronic)
IS  - 0099-2240 (Linking)
VI  - 73
IP  - 16
DP  - 2007 Aug
TI  - Diversity of nitrite reductase genes in "Candidatus Accumulibacter 
      phosphatis"-dominated cultures enriched by flow-cytometric sorting.
PG  - 5331-7
AB  - "Candidatus Accumulibacter phosphatis" is considered a polyphosphate-accumulating 
      organism (PAO) though it has not been isolated yet. To reveal the denitrification 
      ability of this organism, we first concentrated this organism by flow cytometric 
      sorting following fluorescence in situ hybridization (FISH) using specific probes 
      for this organism. The purity of the target cells was about 97% of total cell 
      count in the sorted sample. The PCR amplification of the nitrite reductase genes 
      (nirK and nirS) from unsorted and sorted cells was performed. Although nirK and 
      nirS were amplified from unsorted cells, only nirS was detected from sorted 
      cells, indicating that "Ca. Accumulibacter phosphatis" has nirS. Furthermore, 
      nirS fragments were cloned from unsorted (Ba clone library) and sorted (Bd clone 
      library) cells and classified by restriction fragment length polymorphism 
      analysis. The most dominant clone in clone library Ba, which represented 62% of 
      the total number of clones, was not found in clone library Bd. In contrast, the 
      most dominant clone in clone library Bd, which represented 59% of the total 
      number of clones, represented only 2% of the total number of clones in clone 
      library Ba, indicating that this clone could be that of "Ca. Accumulibacter 
      phosphatis." The sequence of this nirS clone exhibited less than 90% similarity 
      to the sequences of known denitrifying bacteria in the database. The recovery of 
      the nirS genes makes it likely that "Ca. Accumulibacter phosphatis" behaves as a 
      denitrifying PAO capable of utilizing nitrite instead of oxygen as an electron 
      acceptor for phosphorus uptake.
FAU - Miyauchi, Ryuki
AU  - Miyauchi R
AD  - Department of Chemical Engineering, Waseda University, 3-4-1 Ohkubo, Shinjuku-ku, 
      Tokyo 169-8555, Japan.
FAU - Oki, Kazuma
AU  - Oki K
FAU - Aoi, Yoshiteru
AU  - Aoi Y
FAU - Tsuneda, Satoshi
AU  - Tsuneda S
LA  - eng
SI  - GENBANK/AB208081
SI  - GENBANK/AB208082
SI  - GENBANK/AB208083
SI  - GENBANK/AB208084
SI  - GENBANK/AB208085
SI  - GENBANK/AB208086
SI  - GENBANK/AB208087
SI  - GENBANK/AB208088
SI  - GENBANK/AB208089
SI  - GENBANK/AB208090
SI  - GENBANK/AB208091
SI  - GENBANK/AB208092
SI  - GENBANK/AB208093
SI  - GENBANK/AB208094
SI  - GENBANK/AB208095
SI  - GENBANK/AB208096
SI  - GENBANK/AB208097
SI  - GENBANK/AB208098
SI  - GENBANK/AB208099
SI  - GENBANK/AB208100
SI  - GENBANK/AB208101
SI  - GENBANK/AB208102
SI  - GENBANK/AB208103
SI  - GENBANK/AB208104
SI  - GENBANK/AB208105
PT  - Journal Article
DEP - 20070518
PL  - United States
TA  - Appl Environ Microbiol
JT  - Applied and environmental microbiology
JID - 7605801
RN  - 0 (Bacterial Proteins)
RN  - 0 (DNA, Bacterial)
RN  - EC 1.7.- (Nitrite Reductases)
SB  - IM
MH  - Bacteria/classification/enzymology/*genetics
MH  - Bacterial Proteins/*genetics
MH  - Bioreactors/microbiology
MH  - DNA, Bacterial/chemistry/genetics/isolation & purification
MH  - Flow Cytometry/*methods
MH  - In Situ Hybridization, Fluorescence/methods
MH  - Molecular Sequence Data
MH  - Nitrite Reductases/*genetics
MH  - Phylogeny
MH  - Polymerase Chain Reaction
MH  - Polymorphism, Restriction Fragment Length
MH  - Sequence Analysis, DNA
PMC - PMC1950991
EDAT- 2007/05/22 09:00
MHDA- 2007/10/20 09:00
PMCR- 2007/12/01
CRDT- 2007/05/22 09:00
PHST- 2007/05/22 09:00 [pubmed]
PHST- 2007/10/20 09:00 [medline]
PHST- 2007/05/22 09:00 [entrez]
PHST- 2007/12/01 00:00 [pmc-release]
AID - AEM.00175-07 [pii]
AID - 0175-07 [pii]
AID - 10.1128/AEM.00175-07 [doi]
PST - ppublish
SO  - Appl Environ Microbiol. 2007 Aug;73(16):5331-7. doi: 10.1128/AEM.00175-07. Epub 
      2007 May 18.