PMID- 17514404
OWN - NLM
STAT- MEDLINE
DCOM- 20080211
LR  - 20181113
IS  - 1436-2228 (Print)
IS  - 1436-2228 (Linking)
VI  - 9
IP  - 5
DP  - 2007 Sep-Oct
TI  - Diversity of the bacterial communities associated with the azooxanthellate deep 
      water octocorals Leptogorgia minimata, Iciligorgia schrammi, and Swiftia exertia.
PG  - 561-76
AB  - This study examined the microbiota associated with the marine azooxanthellate 
      octocorals Leptogorgia minimata, Swiftia exertia, and Iciligorgia schrammi 
      collected from moderate depths (45 m). Traditional aerobic plate culture, 
      fluorescence in situ hybridization (FISH), and molecular identification of the 
      16S rDNA region were used for this purpose. In general, cultures were found to be 
      selective for Gammaproteobacteria, Alphaproteobacteria, and Firmicutes. 
      Interestingly, FISH counts for Firmicutes in the whole coral (holobiont) were 
      near the detection limit of this assay, representing less than 6% of the total 
      detectable microbiota in all counts. Proteobacteria, especially Alpha- and 
      Gammaproteobacteria, made up the majority of the total microbiota in the 
      holobionts. In addition, the absence of zooxanthellae in these three corals was 
      confirmed by the use of polymerase chain reaction (PCR) and 
      dinoflagellate-specific primers, and spectrophotometric chlorophyll pigment 
      measurements. No evidence of zooxanthellae could be found in any of the corals by 
      either of these techniques. This is the first study examining the microbiota 
      marine octocorals, which grow at moderate depth (40 to 100 m) in the absence of 
      direct sunlight.
FAU - Bruck, Thomas B
AU  - Bruck TB
AD  - Center of Excellence in Biomedical and Marine Biotechnology, Department of 
      Chemistry and Biochemistry, Florida Atlantic University, Boca Raton, FL 33431, 
      USA.
FAU - Bruck, Wolfram M
AU  - Bruck WM
FAU - Santiago-Vazquez, Lory Z
AU  - Santiago-Vazquez LZ
FAU - McCarthy, Peter J
AU  - McCarthy PJ
FAU - Kerr, Russell G
AU  - Kerr RG
LA  - eng
SI  - GENBANK/DQ517183
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PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20070519
PL  - United States
TA  - Mar Biotechnol (NY)
JT  - Marine biotechnology (New York, N.Y.)
JID - 100892712
RN  - 0 (RNA, Ribosomal, 16S)
SB  - IM
MH  - Animals
MH  - Anthozoa/*microbiology
MH  - Bacteria/*classification/*genetics/growth & development/isolation & purification
MH  - Base Sequence
MH  - *Biodiversity
MH  - Colony Count, Microbial
MH  - Dinoflagellida/genetics/isolation & purification
MH  - In Situ Hybridization, Fluorescence/veterinary
MH  - Molecular Sequence Data
MH  - Phylogeny
MH  - RNA, Ribosomal, 16S/*genetics
EDAT- 2007/05/22 09:00
MHDA- 2008/02/12 09:00
CRDT- 2007/05/22 09:00
PHST- 2007/02/10 00:00 [received]
PHST- 2007/03/12 00:00 [accepted]
PHST- 2007/02/10 00:00 [revised]
PHST- 2007/05/22 09:00 [pubmed]
PHST- 2008/02/12 09:00 [medline]
PHST- 2007/05/22 09:00 [entrez]
AID - 10.1007/s10126-007-9009-1 [doi]
PST - ppublish
SO  - Mar Biotechnol (NY). 2007 Sep-Oct;9(5):561-76. doi: 10.1007/s10126-007-9009-1. 
      Epub 2007 May 19.