PMID- 1751450
OWN - NLM
STAT- MEDLINE
DCOM- 19920128
LR  - 20141120
IS  - 1043-4674 (Print)
IS  - 1043-4674 (Linking)
VI  - 3
IP  - 7
DP  - 1991 Jul
TI  - Soluble HTLV-I Tax1 protein stimulates proliferation of human peripheral blood 
      lymphocytes.
PG  - 678-86
AB  - Human T-cell leukemia virus type I (HTLV-I) is associated with two human 
      diseases, adult T-cell leukemia (ATL) and tropical spastic paraparesis/HTLV-I 
      associated myelopathy (TSP/HAM). Lymphocytes from patients with ATL or TSP/HAM 
      display abnormal proliferation properties in culture. Here we report that 
      purified, soluble Tax1 protein can be taken up by, and stimulate proliferation 
      of, uninfected human peripheral blood lymphocytes (PBLs) that have been 
      stimulated with phytohemagglutinin (PHA). Tax1 was 40 to 70% as active as 
      interleukin-2 (IL-2) in stimulating proliferation of PBLs. Heat inactivation, 
      chloroform extraction, and immunoprecipitation with antisera specific for Tax1 
      each abolished the ability of the protein to stimulate lymphocyte proliferation. 
      Tax1 failed to stimulate PBL proliferation in the absence of PHA. After an 
      initial round of cell division, Tax1-treated PBLs exhibited prolonged sensitivity 
      to IL-2-induced proliferation. These results indicate that Tax1 can stimulate 
      lymphocyte proliferation in culture and imply that extracellular Tax1 may be 
      involved in the spontaneous proliferation of TSP/HAM lymphocytes and the 
      IL-2-dependent proliferation of ATL lymphocytes.
FAU - Marriott, S J
AU  - Marriott SJ
AD  - Laboratory of Molecular Virology, National Cancer Institute, Bethesda, MD 20892.
FAU - Lindholm, P F
AU  - Lindholm PF
FAU - Reid, R L
AU  - Reid RL
FAU - Brady, J N
AU  - Brady JN
LA  - eng
PT  - Journal Article
PL  - United States
TA  - New Biol
JT  - The New biologist
JID - 9000976
RN  - 0 (Gene Products, tax)
RN  - 0 (Interleukin-2)
RN  - 0 (Phytohemagglutinins)
RN  - 7V31YC746X (Chloroform)
RN  - EC 2.3.1.28 (Chloramphenicol O-Acetyltransferase)
SB  - IM
MH  - Blotting, Western
MH  - Cell Division/drug effects
MH  - Chloramphenicol O-Acetyltransferase/biosynthesis
MH  - Chloroform/pharmacology
MH  - Drug Interactions
MH  - Gene Expression Regulation, Viral
MH  - Gene Products, tax/pharmacokinetics/*pharmacology
MH  - Hot Temperature/adverse effects
MH  - Humans
MH  - In Vitro Techniques
MH  - Interleukin-2/pharmacology
MH  - Leukemia, T-Cell
MH  - Lymphocyte Activation/*drug effects
MH  - Lymphocytes/metabolism
MH  - Phytohemagglutinins
MH  - Time Factors
MH  - Transcriptional Activation
EDAT- 1991/07/01 00:00
MHDA- 1991/07/01 00:01
CRDT- 1991/07/01 00:00
PHST- 1991/07/01 00:00 [pubmed]
PHST- 1991/07/01 00:01 [medline]
PHST- 1991/07/01 00:00 [entrez]
PST - ppublish
SO  - New Biol. 1991 Jul;3(7):678-86.