PMID- 17516843
OWN - NLM
STAT- MEDLINE
DCOM- 20070608
LR  - 20161124
IS  - 1431-6730 (Print)
IS  - 1431-6730 (Linking)
VI  - 388
IP  - 5
DP  - 2007 May
TI  - Calcium signalling in the regulation of PGC-1alpha, PDK4 and HKII mRNA 
      expression.
PG  - 481-8
AB  - The role of calcium signalling and specific intracellular calcium signalling 
      pathways in regulating skeletal muscle tissue peroxisome proliferator-activated 
      receptor gamma co-activator (PGC)-1alpha, hexokinase (HK)II and pyruvate 
      dehydrogenase kinase (PDK)4 mRNA was examined. Cultured primary rat skeletal 
      muscle cells were incubated for 6 h in caffeine or ionomycin. Because PGC-1alpha 
      mRNA clearly showed greater induction with ionomycin, the latter was chosen for 
      the main experiments, whereby cells were incubated for 6 h with either ionomycin 
      alone or in combination with either cyclosporin A or KN-62. The PGC-1alpha mRNA 
      level was increased (p<0.05) approximately six-fold and HKII mRNA content 
      approximately two-fold by ionomycin relative to the corresponding controls, 
      whereas the PDK4 mRNA content remained unaffected. Cyclosporin A abolished 
      (p<0.05) and KN-62 reduced (p<0.1) the ionomycin-induced increase in PGC-1alpha 
      mRNA. Electrical stimulation of in vitro incubated rat EDL muscle increased 
      (p<0.05) PGC-1alpha mRNA by 2.2-fold after 4 h of recovery relative to a resting 
      control, and this increase was absent when muscles were incubated with KN-62 or 
      cyclosporin A. The present data strongly suggest that calcium signalling is 
      involved in regulating the PGC-1alpha and HKII genes, but not PDK4. Both 
      calcineurin and CaMK signalling seem to be involved in the calcium- and 
      contraction-mediated PGC-1alpha up-regulation in skeletal muscle.
FAU - Kusuhara, Keiko
AU  - Kusuhara K
AD  - Copenhagen Muscle Research Centre and Centre of Inflammation and Metabolism, The 
      August Krogh Building, Department of Molecular Biology, University of Copenhagen, 
      Copenhagen O, Denmark.
FAU - Madsen, Klavs
AU  - Madsen K
FAU - Jensen, Lotte
AU  - Jensen L
FAU - Hellsten, Ylva
AU  - Hellsten Y
FAU - Pilegaard, Henriette
AU  - Pilegaard H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Biol Chem
JT  - Biological chemistry
JID - 9700112
RN  - 0 (Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha)
RN  - 0 (Ppargc1a protein, rat)
RN  - 0 (RNA, Messenger)
RN  - 0 (RNA-Binding Proteins)
RN  - 0 (Transcription Factors)
RN  - 3G6A5W338E (Caffeine)
RN  - 56092-81-0 (Ionomycin)
RN  - 63HM46XPOW (KN 62)
RN  - 83HN0GTJ6D (Cyclosporine)
RN  - 84477-87-2 (1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine)
RN  - EC 2.7.- (Protein Kinases)
RN  - EC 2.7.1.- (pyruvate dehydrogenase kinase 4)
RN  - EC 2.7.1.1 (Hexokinase)
SB  - IM
MH  - 1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine/analogs & derivatives/pharmacology
MH  - Animals
MH  - Caffeine/pharmacology
MH  - *Calcium Signaling
MH  - Cells, Cultured
MH  - Cyclosporine/pharmacology
MH  - Electric Stimulation
MH  - Gene Expression Regulation/drug effects/*genetics
MH  - Hexokinase/classification/genetics/*metabolism
MH  - Ionomycin/pharmacology
MH  - Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha
MH  - Protein Kinases/genetics/*metabolism
MH  - RNA, Messenger/genetics
MH  - RNA-Binding Proteins/genetics/*metabolism
MH  - Rats
MH  - Rats, Wistar
MH  - Transcription Factors/genetics/*metabolism
EDAT- 2007/05/23 09:00
MHDA- 2007/06/09 09:00
CRDT- 2007/05/23 09:00
PHST- 2007/05/23 09:00 [pubmed]
PHST- 2007/06/09 09:00 [medline]
PHST- 2007/05/23 09:00 [entrez]
AID - 10.1515/BC.2007.052 [doi]
PST - ppublish
SO  - Biol Chem. 2007 May;388(5):481-8. doi: 10.1515/BC.2007.052.