PMID- 17518608
OWN - NLM
STAT- MEDLINE
DCOM- 20070622
LR  - 20220317
IS  - 1076-3279 (Print)
IS  - 1076-3279 (Linking)
VI  - 13
IP  - 3
DP  - 2007 Mar
TI  - In vivo osteogenic potential of human adipose-derived stem cells/poly 
      lactide-co-glycolic acid constructs for bone regeneration in a rat critical-sized 
      calvarial defect model.
PG  - 619-27
AB  - Recent studies suggest that human adipose tissue contains pluripotent stem cells, 
      which are similar to bone marrow-derived stem cells. The objective of the present 
      study was to assess the effect in bone regenerating capability of human 
      adipose-derived stem cells (ADSCs) cultured in osteogenic media layered over poly 
      lactide-co-glycolic acid (PLGA) and implanted in a critical nude rat calvarial 
      defect. Twenty-seven nude rats were randomized into 3 groups (n = 9): 1) PLGA 
      alone (control), 2) PLGA with undifferentiated ADSCs, and 3) PLGA with 
      differentiated ADSCs. These 3 groups were divided into 9 subgroups (n = 3) 
      according to in vitro pre-cultured periods (day 1 pre-culture (Group1), day 7 
      pre-culture (Group2), and day 14 pre-culture (Group3)) before implantation. An 8 
      mm critical-size circular calvarial defect was made in each nude rat. Specimens 
      were harvested at 12 weeks post-implantation and evaluated radiographically and 
      histologically. Radiodensitometric analysis revealed significantly higher bone 
      growth in implants pre-cultured in osteogenic media for 14 days for Group 3. 
      Histomorphometric analysis demonstrated that Groups 2 and 3 had bone formation 
      filling 35% to 72% of the area of the defect after transplantation with cells 
      that had been pre-cultured for 14 days. Constructs with differentiated ADSCs 
      (Group 3) had noticeably more maximal and robust bone tissue regeneration than 
      constructs with undifferentiated ADSCs (Group 2). These data provide evidence 
      that constructs or implants made of PLGA and osteogenically differentiated ADSCs 
      pre-cultured for 14 days before transplantation have better, more-robust bone 
      regeneration capability in critical-sized skeletal defects than constructs with 
      undifferentiated ADSCs. Human adipose derived stem cells can therefore be used as 
      seed cells to construct tissue-engineered bone.
FAU - Yoon, Eulsik
AU  - Yoon E
AD  - Aesthetic and Plastic Surgery Institute, Korea University Ansan Hospital, 
      Danwon-gu, Ansan, Kyunggi-do, Korea.
FAU - Dhar, Sanjay
AU  - Dhar S
FAU - Chun, Daniel E
AU  - Chun DE
FAU - Gharibjanian, Nareg A
AU  - Gharibjanian NA
FAU - Evans, Gregory R D
AU  - Evans GR
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Tissue Eng
JT  - Tissue engineering
JID - 9505538
RN  - 0 (Biocompatible Materials)
RN  - 0 (Glycolates)
RN  - 1SIA8062RS (Polylactic Acid-Polyglycolic Acid Copolymer)
RN  - 26009-03-0 (Polyglycolic Acid)
RN  - 33X04XA5AT (Lactic Acid)
SB  - IM
MH  - Adipose Tissue/*cytology/transplantation
MH  - Animals
MH  - *Biocompatible Materials
MH  - Bone Regeneration/*physiology
MH  - Cells, Cultured
MH  - *Glycolates
MH  - Humans
MH  - Lactic Acid
MH  - Osteogenesis/*physiology
MH  - Polyglycolic Acid
MH  - Polylactic Acid-Polyglycolic Acid Copolymer
MH  - Rats
MH  - Rats, Nude
MH  - Skull/injuries/*physiology/surgery
MH  - *Stem Cell Transplantation/methods
EDAT- 2007/05/24 09:00
MHDA- 2007/06/23 09:00
CRDT- 2007/05/24 09:00
PHST- 2007/05/24 09:00 [pubmed]
PHST- 2007/06/23 09:00 [medline]
PHST- 2007/05/24 09:00 [entrez]
AID - 10.1089/ten.2006.0102 [doi]
PST - ppublish
SO  - Tissue Eng. 2007 Mar;13(3):619-27. doi: 10.1089/ten.2006.0102.