PMID- 17518647
OWN - NLM
STAT- MEDLINE
DCOM- 20070619
LR  - 20071203
IS  - 1076-3279 (Print)
IS  - 1076-3279 (Linking)
VI  - 12
IP  - 10
DP  - 2006 Oct
TI  - Templated agarose scaffolds support linear axonal regeneration.
PG  - 2777-87
AB  - While several strategies can stimulate axonal regeneration within a site of 
      spinal cord injury, the growth of axons is generally disorganized and random. 
      Biocompatible scaffolds that guide and maintain the native organization of axons 
      regenerating through an injury site could be of importance in enhancing recovery 
      of the nervous system after injury. Here we report a novel fabrication process 
      for templated agarose nerve guidance scaffolds composed of uniaxial channels of 
      precise diameter and wall thickness extending through their full length. When 
      tested in an in vivo model of spinal cord injury, scaffolds exhibit excellent 
      integration with host tissue and support linear axonal growth through their 
      channels. Further, when loaded with bone marrow stromal cells genetically 
      engineered to secrete brain-derived neurotrophic factor (BDNF), the number of 
      linear penetrating axons is significantly enhanced. The templating process can be 
      useful in fabricating nerve guidance scaffolds for both central and peripheral 
      nerve injuries, or any materials application requiring a precise array of 
      linearly oriented channels.
FAU - Stokols, Shula
AU  - Stokols S
AD  - Department of Bioengineering, University of California-San Diego, La Jolla, 
      California 92093, USA.
FAU - Sakamoto, Jeff
AU  - Sakamoto J
FAU - Breckon, Chris
AU  - Breckon C
FAU - Holt, Todd
AU  - Holt T
FAU - Weiss, James
AU  - Weiss J
FAU - Tuszynski, Mark H
AU  - Tuszynski MH
LA  - eng
GR  - NS09881/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - Tissue Eng
JT  - Tissue engineering
JID - 9505538
RN  - 9012-36-6 (Sepharose)
SB  - IM
MH  - Animals
MH  - Axons/*physiology/*ultrastructure
MH  - Cells, Cultured
MH  - Female
MH  - Guided Tissue Regeneration/*methods
MH  - Nerve Regeneration/*physiology
MH  - Rats
MH  - Rats, Inbred F344
MH  - Sepharose/*chemistry
MH  - Tissue Engineering/*methods
EDAT- 2007/05/24 09:00
MHDA- 2007/06/20 09:00
CRDT- 2007/05/24 09:00
PHST- 2007/05/24 09:00 [pubmed]
PHST- 2007/06/20 09:00 [medline]
PHST- 2007/05/24 09:00 [entrez]
AID - 10.1089/ten.2006.12.2777 [doi]
PST - ppublish
SO  - Tissue Eng. 2006 Oct;12(10):2777-87. doi: 10.1089/ten.2006.12.2777.