PMID- 17518658
OWN - NLM
STAT- MEDLINE
DCOM- 20070619
LR  - 20181201
IS  - 1076-3279 (Print)
IS  - 1076-3279 (Linking)
VI  - 12
IP  - 10
DP  - 2006 Oct
TI  - Sequential growth factor stimulation of bone marrow stromal cells in extended 
      culture.
PG  - 2905-12
AB  - The sequence of applied biochemical stimulation in developing ligament tissue 
      cultures in vitro offers the potential to intricately control cell behavior 
      following the template of native tissue development. Previous studies have 
      identified and enhanced ligament tissue development as defined by matrix 
      in-growth, upregulation of mRNA transcripts for metalloproteinase-2 (MMP-2), 
      collagen types I and III, and collagen type I production. We hypothesize that 
      sequential application of growth factors through extended culture will reinforce 
      the effectiveness of basic fibroblast growth factor and transforming growth 
      factor beta (bFGF/TGF-beta) as the optimal growth factor regimen. Bone marrow 
      stromal cells (BMSCs) were seeded on RGD-coupled silk fiber matrices and cultured 
      in bFGF, epidermal growth factor (EGF), or growth factor-free control for the 
      first 5 days of culture. On day 5, cultures were stimulated with TGF-beta 
      supplemented medium for a total of 28 days. Results indicated enhanced matrix 
      in-growth and collagen type I produced with extended culture, most notably in 
      mitogen / TGF-beta-stimulated cultures. Matrix development attained through 
      extended static culture will support future study leading to the transition and 
      addition of mechanical stimulation for optimized ligament tissue production.
FAU - Moreau, Jodie
AU  - Moreau J
AD  - Department of Biomedical Engineering, Tufts University, Medford, Massachusetts 
      02155, USA.
FAU - Chen, Jingsong
AU  - Chen J
FAU - Kaplan, David
AU  - Kaplan D
FAU - Altman, Gregory
AU  - Altman G
LA  - eng
GR  - R01 AR46563-01/AR/NIAMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PL  - United States
TA  - Tissue Eng
JT  - Tissue engineering
JID - 9505538
RN  - 0 (Drug Combinations)
RN  - 0 (Extracellular Matrix Proteins)
RN  - 0 (Intercellular Signaling Peptides and Proteins)
SB  - IM
MH  - Cell Culture Techniques/*methods
MH  - Cell Proliferation/drug effects
MH  - Cell Survival/drug effects
MH  - Cells, Cultured
MH  - Dose-Response Relationship, Drug
MH  - Drug Combinations
MH  - Extracellular Matrix/drug effects/*physiology
MH  - Extracellular Matrix Proteins/*metabolism
MH  - Humans
MH  - Intercellular Signaling Peptides and Proteins/*administration & dosage
MH  - Mesenchymal Stem Cells/*cytology/drug effects/*physiology
MH  - Tissue Engineering/*methods
EDAT- 2007/05/24 09:00
MHDA- 2007/06/20 09:00
CRDT- 2007/05/24 09:00
PHST- 2007/05/24 09:00 [pubmed]
PHST- 2007/06/20 09:00 [medline]
PHST- 2007/05/24 09:00 [entrez]
AID - 10.1089/ten.2006.12.2905 [doi]
PST - ppublish
SO  - Tissue Eng. 2006 Oct;12(10):2905-12. doi: 10.1089/ten.2006.12.2905.