PMID- 17518704
OWN - NLM
STAT- MEDLINE
DCOM- 20070731
LR  - 20220411
IS  - 1076-3279 (Print)
IS  - 1076-3279 (Linking)
VI  - 13
IP  - 6
DP  - 2007 Jun
TI  - Expanded adipose-derived stem cells suppress mixed lymphocyte reaction by 
      secretion of prostaglandin E2.
PG  - 1185-95
AB  - Multipotent mesenchymal stem cells (MSCs) in adult tissue are known to be less 
      immunogenic and immunosuppressive. Previous study showed that primary cultures of 
      human adipose-derived stem cells (ADSCs) shared their immunomodulatory properties 
      with other MSCs. However, whether passaged human ADSCs can retain their 
      immunomodulatory effect after in vitro expansion remains unknown. In addition, 
      the mechanism of ADSC-mediated immunomodulatory effect remains to be elucidated. 
      This study aimed to investigate these issues by using passaged human ADSCs as an 
      in vitro study model. Flow cytometry showed that passaged ADSCs expressed human 
      leukocyte antigen (HLA) class I but not class II molecules, which could be 
      induced to express to a high level with interferon-gamma (IFN-gamma) treatment. 
      The study found that passaged ADSCs could not elicit lymphocyte proliferation 
      after co-culturing with them, even after IFN-gamma treatment. In addition, either 
      IFN-gamma-treated or non-treated ADSCs could inhibit phytohemagglutinin 
      (PHA)-stimulated lymphocyte proliferation. Moreover, passaged ADSCs could serve 
      as the third-party cells to inhibited two-way mixed lymphocyte reaction (MLR). 
      Further study using a transwell system also showed that this type of 
      immunosuppressive effect was not cell-cell contact dependent. In defining 
      possible soluble factors, we found that passaged ADSCs significantly increased 
      their secretion of prostaglandin E2 (PGE2), but not transforming growth 
      factor-beta (TGF-beta) and hepatocyte growth factor (HGF), when they were 
      co-cultured with MLR. Furthermore, the result demonstrated that only PGE2 
      production inhibitor indomethacine, but not TGF-beta- and HGF-neutralizing 
      antibodies, could significantly counteract ADSC-mediated suppression on 
      allogeneic lymphocyte proliferation. These results indicated that in vitro 
      expanded ADSCs retain low immunogenicity and immunosuppressive effect, and PGE2 
      might be the major soluble factor involved in the in vitro inhibition of 
      allogeneic lymphocyte reaction.
FAU - Cui, Lei
AU  - Cui L
AD  - Department of Plastic and Reconstructive Surgery, 9th People's Hospital, School 
      of Medicine, Shanghai Jiao Tong University, Shanghai, People's Republic of China.
FAU - Yin, Shuo
AU  - Yin S
FAU - Liu, Wei
AU  - Liu W
FAU - Li, Ningli
AU  - Li N
FAU - Zhang, Wenjie
AU  - Zhang W
FAU - Cao, Yilin
AU  - Cao Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Tissue Eng
JT  - Tissue engineering
JID - 9505538
RN  - 0 (Immunologic Factors)
RN  - K7Q1JQR04M (Dinoprostone)
SB  - IM
MH  - Adipocytes/*cytology/*immunology
MH  - Amnion/*cytology/*immunology
MH  - Cell Communication/immunology
MH  - Cell Differentiation
MH  - Cells, Cultured
MH  - Dinoprostone/*immunology
MH  - Dose-Response Relationship, Drug
MH  - Humans
MH  - Immunologic Factors/*immunology
MH  - Lymphocyte Activation/*immunology
MH  - Lymphocytes/immunology
MH  - Stem Cells/*cytology/*immunology
MH  - Tissue Engineering/methods
EDAT- 2007/05/24 09:00
MHDA- 2007/08/01 09:00
CRDT- 2007/05/24 09:00
PHST- 2007/05/24 09:00 [pubmed]
PHST- 2007/08/01 09:00 [medline]
PHST- 2007/05/24 09:00 [entrez]
AID - 10.1089/ten.2006.0315 [doi]
PST - ppublish
SO  - Tissue Eng. 2007 Jun;13(6):1185-95. doi: 10.1089/ten.2006.0315.