PMID- 17519568
OWN - NLM
STAT- MEDLINE
DCOM- 20070906
LR  - 20220410
IS  - 1229-845X (Print)
IS  - 1976-555X (Electronic)
IS  - 1229-845X (Linking)
VI  - 8
IP  - 2
DP  - 2007 Jun
TI  - PCR-based detection of genes encoding virulence determinants in Staphylococcus 
      aureus from bovine subclinical mastitis cases.
PG  - 151-4
AB  - The present study was carried out to genotypically characterize Staphylococcus 
      aureus (S. aureus) isolated from bovine mastitis cases. A total of 37 strains of 
      S. aureus were isolated during processing of 552 milk samples from 140 cows. The 
      S. aureus strains were characterized phenotypically, and were further 
      characterized genotypically by polymerase chain reaction using oligonucleotide 
      primers that amplified genes encoding coagulase (coa), clumping factor (clfA), 
      thermonuclease (nuc), enterotoxin A (entA), and the gene segments encoding the 
      immunoglobulin G binding region and the X region of protein A gene spa. All of 
      the isolates yielded an amplicon with a size of approximately 1,042 bp of the 
      clfA gene. The amplification of the polymorphic spa gene segment encoding the 
      immunoglobulin G binding region was observed in 34 isolates and X-region binding 
      was detected in 26 isolates. Amplification of the coa gene yielded three 
      different products in 20, 10, and 7 isolates. The amplification of the 
      thermonuclease gene, nuc, was observed in 36 out of 37 isolates. All of the 
      samples were negative for the entA gene. The phenotypic and genotypic findings of 
      the present strategies might provide an understanding of the distribution of the 
      prevalent S. aureus clones among bovine mastitis isolates, and might aid in the 
      development of steps to control S. aureus infections in dairy herds.
FAU - Kalorey, Dewanand Rajaram
AU  - Kalorey DR
AD  - Department of Microbiology, Nagpur Veterinary College, Maharashtra Animal and 
      Fishery Sciences University, Nagpur, Maharashtra, India. 
      dewanandkalorey@rediffmail.com
FAU - Shanmugam, Yuvaraj
AU  - Shanmugam Y
FAU - Kurkure, Nitin Vasantrao
AU  - Kurkure NV
FAU - Chousalkar, Kapil Kamalakarrao
AU  - Chousalkar KK
FAU - Barbuddhe, Sukhadeo Baliram
AU  - Barbuddhe SB
LA  - eng
PT  - Journal Article
PL  - Korea (South)
TA  - J Vet Sci
JT  - Journal of veterinary science
JID - 100964185
RN  - 0 (Bacterial Proteins)
RN  - 0 (ClfA protein, Staphylococcus aureus)
RN  - 0 (Coagulase)
RN  - 0 (DNA, Bacterial)
RN  - 0 (Virulence Factors)
RN  - 0 (nuc protein, staphylococcus)
RN  - EC 3.1.- (Endonucleases)
RN  - EC 3.1.31.1 (Micrococcal Nuclease)
SB  - IM
MH  - Animals
MH  - Bacterial Proteins/chemistry/genetics
MH  - Cattle
MH  - Coagulase/chemistry/genetics
MH  - DNA, Bacterial/chemistry/genetics
MH  - Endonucleases/chemistry/genetics
MH  - Female
MH  - Mastitis, Bovine/*microbiology
MH  - Micrococcal Nuclease/chemistry/genetics
MH  - Milk/microbiology
MH  - Polymerase Chain Reaction/veterinary
MH  - Staphylococcal Infections/microbiology/*veterinary
MH  - Staphylococcus aureus/*genetics/pathogenicity
MH  - Virulence Factors/chemistry/*genetics
PMC - PMC2872713
EDAT- 2007/05/24 09:00
MHDA- 2007/09/07 09:00
PMCR- 2007/06/01
CRDT- 2007/05/24 09:00
PHST- 2007/05/24 09:00 [pubmed]
PHST- 2007/09/07 09:00 [medline]
PHST- 2007/05/24 09:00 [entrez]
PHST- 2007/06/01 00:00 [pmc-release]
AID - 200706151 [pii]
AID - 10.4142/jvs.2007.8.2.151 [doi]
PST - ppublish
SO  - J Vet Sci. 2007 Jun;8(2):151-4. doi: 10.4142/jvs.2007.8.2.151.