PMID- 17521721
OWN - NLM
STAT- MEDLINE
DCOM- 20080205
LR  - 20181201
IS  - 0145-305X (Print)
IS  - 0145-305X (Linking)
VI  - 31
IP  - 12
DP  - 2007
TI  - Adenosine arrests apoptosis in lymphocytes but not in phagocytes from primary 
      leucocyte cultures of the teleost fish, Sparus aurata L.
PG  - 1233-41
AB  - Adenosine (A) and its derivatives have important biological functions, including 
      the inhibition of immune responses and apoptosis induction. The aim of this work 
      was to investigate whether A and N(6)-cyclohexyladenosine (CHA) regulate 
      apoptosis in leukocyte cultures from the teleost, gilthead seabream (Sparus 
      aurata L.) by flow cytometry using a double fluorescence staining method. Head 
      kidney leukocytes (HKLs) were cultured for 0, 24 or 48h. The kinetics of 
      FDA(+)/PI(-) (viable), FDA(-)/PI(-) (apoptotic) and FDA(-)/PI(+) (necrotic) 
      leukocyte subpopulations were followed during the culture process. Apoptosis was 
      induced by addition of resveratrol or staurosporine to the culture media and the 
      study was validated by transmission electron microscopy. A and CHA did not induce 
      but decreased apoptosis of seabream HKLs, in particular HK lymphocytes (L), after 
      24h in culture. Results raise the question whether apoptosis is differently 
      modulated by purine nucleosides in fish L and phagocytes and also in fish 
      compared to mammalian cells.
FAU - Salinas, Irene
AU  - Salinas I
AD  - Fish Innate Immune System Group, Department of Cell Biology, Faculty of Biology, 
      Campus de Espinardo, University of Murcia, 30100 Murcia, Spain.
FAU - Rodriguez, Alejandro
AU  - Rodriguez A
FAU - Meseguer, Jose
AU  - Meseguer J
FAU - Esteban, Maria Angeles
AU  - Esteban MA
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20070504
PL  - United States
TA  - Dev Comp Immunol
JT  - Developmental and comparative immunology
JID - 7708205
RN  - 0 (Stilbenes)
RN  - 36396-99-3 (N(6)-cyclohexyladenosine)
RN  - H88EPA0A3N (Staurosporine)
RN  - K72T3FS567 (Adenosine)
RN  - Q369O8926L (Resveratrol)
SB  - IM
MH  - Adenosine/*analogs & derivatives/pharmacology/*physiology
MH  - Animals
MH  - *Apoptosis/drug effects
MH  - Cells, Cultured
MH  - Lymphocytes/*cytology/drug effects/ultrastructure
MH  - Microscopy, Electron, Transmission
MH  - Phagocytes/*cytology/drug effects/ultrastructure
MH  - Resveratrol
MH  - Sea Bream/blood/*physiology
MH  - Staurosporine/pharmacology
MH  - Stilbenes/pharmacology
EDAT- 2007/05/25 09:00
MHDA- 2008/02/06 09:00
CRDT- 2007/05/25 09:00
PHST- 2006/12/18 00:00 [received]
PHST- 2007/03/28 00:00 [revised]
PHST- 2007/03/30 00:00 [accepted]
PHST- 2007/05/25 09:00 [pubmed]
PHST- 2008/02/06 09:00 [medline]
PHST- 2007/05/25 09:00 [entrez]
AID - S0145-305X(07)00065-1 [pii]
AID - 10.1016/j.dci.2007.03.014 [doi]
PST - ppublish
SO  - Dev Comp Immunol. 2007;31(12):1233-41. doi: 10.1016/j.dci.2007.03.014. Epub 2007 
      May 4.