PMID- 17522215
OWN - NLM
STAT- MEDLINE
DCOM- 20070926
LR  - 20181201
IS  - 0022-538X (Print)
IS  - 1098-5514 (Electronic)
IS  - 0022-538X (Linking)
VI  - 81
IP  - 15
DP  - 2007 Aug
TI  - Epstein-Barr virus induces MCP-1 secretion by human monocytes via TLR2.
PG  - 8016-24
AB  - Epstein-Barr virus (EBV) is a gammaherpesvirus infecting the majority of the 
      human adult population in the world. TLR2, a member of the Toll-like receptor 
      (TLR) family, has been implicated in the immune responses to different viruses 
      including members of the herpesvirus family, such as human cytomegalovirus, 
      herpes simplex virus type 1, and varicella-zoster virus. In this report, we 
      demonstrate that infectious and UV-inactivated EBV virions lead to the activation 
      of NF-kappaB through TLR2 using HEK293 cells cotransfected with TLR2-expressing 
      vector along with NF-kappaB-Luc reporter plasmid. NF-kappaB activation in 
      HEK293-TLR2 cells (HEK293 cells transfected with TLR2) by EBV was not enhanced by 
      the presence of CD14. The effect of EBV was abrogated by pretreating HEK293-TLR2 
      cells with blocking anti-TLR2 antibodies or by preincubating viral particles with 
      neutralizing anti-EBV antibodies 72A1. In addition, EBV infection of primary 
      human monocytes induced the release of MCP-1 (monocyte chemotactic protein 1), 
      and the use of small interfering RNA targeting TLR2 significantly reduced such a 
      chemokine response to EBV. Taken together, these results indicate that TLR2 may 
      be an important pattern recognition receptor in the immune response directed 
      against EBV infection.
FAU - Gaudreault, Eric
AU  - Gaudreault E
AD  - Viral Immunology Laboratory, CHUL Research Center (CHUQ), 2705 boul. Laurier, 
      Room T 1-49, Quebec, QC, Canada G1V 4G2.
FAU - Fiola, Stephanie
AU  - Fiola S
FAU - Olivier, Martin
AU  - Olivier M
FAU - Gosselin, Jean
AU  - Gosselin J
LA  - eng
PT  - Journal Article
DEP - 20070523
PL  - United States
TA  - J Virol
JT  - Journal of virology
JID - 0113724
RN  - 0 (CCL2 protein, human)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Lipopolysaccharide Receptors)
RN  - 0 (NF-kappa B)
RN  - 0 (RNA, Small Interfering)
RN  - 0 (Toll-Like Receptor 2)
SB  - IM
MH  - Adult
MH  - Cell Line
MH  - Chemokine CCL2/immunology/*metabolism
MH  - Genes, Reporter
MH  - Herpesvirus 4, Human/*immunology
MH  - Humans
MH  - Lipopolysaccharide Receptors/immunology
MH  - Monocytes/cytology/*immunology/*virology
MH  - NF-kappa B/immunology
MH  - RNA, Small Interfering/genetics/metabolism
MH  - Toll-Like Receptor 2/genetics/*immunology
MH  - Virion/immunology
PMC - PMC1951286
EDAT- 2007/05/25 09:00
MHDA- 2007/09/27 09:00
PMCR- 2007/12/01
CRDT- 2007/05/25 09:00
PHST- 2007/05/25 09:00 [pubmed]
PHST- 2007/09/27 09:00 [medline]
PHST- 2007/05/25 09:00 [entrez]
PHST- 2007/12/01 00:00 [pmc-release]
AID - JVI.00403-07 [pii]
AID - 0403-07 [pii]
AID - 10.1128/JVI.00403-07 [doi]
PST - ppublish
SO  - J Virol. 2007 Aug;81(15):8016-24. doi: 10.1128/JVI.00403-07. Epub 2007 May 23.