PMID- 17523947
OWN - NLM
STAT- MEDLINE
DCOM- 20070710
LR  - 20191210
IS  - 0300-9475 (Print)
IS  - 0300-9475 (Linking)
VI  - 65
IP  - 6
DP  - 2007 Jun
TI  - Biochip array-based analysis of plasma cytokines in HIV patients with 
      immunological and virological discordance.
PG  - 549-54
AB  - Assessment of cytokines in body fluids or cells provides important information in 
      understanding the disease process and designing treatment strategies. Recent 
      introduction of antibody-based protein arrays have provided investigators 
      simultaneous and specific detection of multiple analytes in a single sample using 
      minimum volumes. In this study, we used a biochip array system capable of 
      measuring 12 cytokines and growth factors (IL-2, IL-4, IL-6, IL-8, IL-10, 
      IL-1alpha, IL-1beta, IFN-gamma, TNF-alpha, monocyte chemoattractant protein-1 
      (MCP-1), vascular endothelial growth factor (VEGF) and epidermal growth factor 
      (EGF)) in HIV patients with immunological and virological discordance 
      (discordant) to find out differences if any, in their plasma cytokine profiles 
      when compared with concordant HIV-infected individuals. A sandwich 
      chemiluminescent assay was performed with plasma specimens of 110 HIV patients 
      (55 discordant, 55 concordant) and 22 normal healthy individuals followed by 
      enzyme-linked immunosorbent assay (ELISA) to the confirm levels of cytokines and 
      growth factors that showed significant differences in the two groups. The 
      discordant HIV patients showed significantly higher levels of plasma VEGF (P = 
      0.001) and EGF (P = 0.034) levels when compared with concordant patients. 
      Overall, the patients showed significantly higher levels of TNF-alpha, MCP-1 and 
      VEGF when compared with the normal healthy controls (P < 0.05). ELISA for VEGF (P 
      < 0.001) and EGF (P = 0.004) confirmed the comparison obtained with biochip 
      array, between the discordant and concordant patients. The results of cytokine 
      quantitation by biochip array and ELISA confirmed that this technology is not 
      only comparable but also has a good potential in the future applications 
      involving measurement of multiple cytokines with limiting specimens.
FAU - Sachdeva, N
AU  - Sachdeva N
AD  - Laboratory for Clinical and Biological Studies, University of Miami-Miller School 
      of Medicine, Miami, FL 33136, USA.
FAU - Yoon, H S
AU  - Yoon HS
FAU - Oshima, K
AU  - Oshima K
FAU - Garcia, D
AU  - Garcia D
FAU - Goodkin, K
AU  - Goodkin K
FAU - Asthana, D
AU  - Asthana D
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PL  - England
TA  - Scand J Immunol
JT  - Scandinavian journal of immunology
JID - 0323767
RN  - 0 (Cytokines)
RN  - 0 (Vascular Endothelial Growth Factors)
RN  - 62229-50-9 (Epidermal Growth Factor)
SB  - IM
MH  - Acquired Immunodeficiency Syndrome/immunology/physiopathology
MH  - Adult
MH  - Antiretroviral Therapy, Highly Active
MH  - Cross-Sectional Studies
MH  - Cytokines/*blood
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Epidermal Growth Factor/blood
MH  - Female
MH  - HIV Infections/drug therapy/*immunology
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - Plasma/chemistry/immunology
MH  - *Protein Array Analysis
MH  - Reference Values
MH  - Vascular Endothelial Growth Factors/blood
EDAT- 2007/05/26 09:00
MHDA- 2007/07/11 09:00
CRDT- 2007/05/26 09:00
PHST- 2007/05/26 09:00 [pubmed]
PHST- 2007/07/11 09:00 [medline]
PHST- 2007/05/26 09:00 [entrez]
AID - SJI1906 [pii]
AID - 10.1111/j.1365-3083.2007.01906.x [doi]
PST - ppublish
SO  - Scand J Immunol. 2007 Jun;65(6):549-54. doi: 10.1111/j.1365-3083.2007.01906.x.