PMID- 17525979
OWN - NLM
STAT- MEDLINE
DCOM- 20071120
LR  - 20070820
IS  - 1932-8451 (Print)
IS  - 1932-8451 (Linking)
VI  - 67
IP  - 11
DP  - 2007 Sep 15
TI  - Activation of Matrix Metalloproteinase-3 is altered at the frog neuromuscular 
      junction following changes in synaptic activity.
PG  - 1488-97
AB  - The extracellular matrix surrounding the neuromuscular junction is a highly 
      specialized and dynamic structure. Matrix Metalloproteinases are enzymes that 
      sculpt the extracellular matrix. Since synaptic activity is critical to the 
      structure and function of this synapse, we investigated whether changes in 
      synaptic activity levels could alter the activity of Matrix Metalloproteinases at 
      the neuromuscular junction. In particular, we focused on Matrix Metalloproteinase 
      3 (MMP3), since antibodies to MMP3 recognize molecules at the frog neuromuscular 
      junction, and MMP3 cleaves a number of synaptic basal lamina molecules, including 
      agrin. Here we show that the fluorogenic compound (M2300) can be used to perform 
      in vivo proteolytic imaging of the frog neuromuscular junction to directly 
      measure the activity state of MMP3. Application of this compound reveals that 
      active MMP3 is concentrated at the normal frog neuromuscular junction, and is 
      tightly associated with the terminal Schwann cell. Blocking presynaptic activity 
      via denervation, or TTX nerve blockade, results in a decreased level of active 
      MMP3 at the neuromuscular junction. The loss of active MMP3 at the neuromuscular 
      junction in denervated muscles can result from decreased activation of pro-MMP3, 
      or it could result from increased inhibition of MMP3. These results support the 
      hypothesis that changes in synaptic activity can alter the level of active MMP3 
      at the neuromuscular junction.
CI  - (c) 2007 Wiley Periodicals, Inc.
FAU - VanSaun, M
AU  - VanSaun M
AD  - Department of Anatomy and Cell Biology, University of Kansas Medical Center, 
      Kansas City, KS 66160, USA.
FAU - Humburg, B C
AU  - Humburg BC
FAU - Arnett, M G
AU  - Arnett MG
FAU - Pence, M
AU  - Pence M
FAU - Werle, M J
AU  - Werle MJ
LA  - eng
GR  - NS33320/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PL  - United States
TA  - Dev Neurobiol
JT  - Developmental neurobiology
JID - 101300215
RN  - 0 (Fluorescent Dyes)
RN  - 0 (Sodium Channel Blockers)
RN  - 4368-28-9 (Tetrodotoxin)
RN  - EC 3.4.24.17 (Matrix Metalloproteinase 3)
SB  - IM
MH  - Animals
MH  - Enzyme Activation/physiology
MH  - Extracellular Matrix/*metabolism
MH  - Fluorescent Dyes
MH  - Matrix Metalloproteinase 3/*metabolism
MH  - Neuromuscular Junction/cytology/drug effects/*enzymology
MH  - Neuronal Plasticity/physiology
MH  - Rana pipiens/anatomy & histology/*physiology
MH  - Schwann Cells/enzymology
MH  - Sodium Channel Blockers/pharmacology
MH  - Staining and Labeling/methods
MH  - Synaptic Transmission/drug effects/*physiology
MH  - Tetrodotoxin/pharmacology
EDAT- 2007/05/26 09:00
MHDA- 2007/12/06 09:00
CRDT- 2007/05/26 09:00
PHST- 2007/05/26 09:00 [pubmed]
PHST- 2007/12/06 09:00 [medline]
PHST- 2007/05/26 09:00 [entrez]
AID - 10.1002/dneu.20523 [doi]
PST - ppublish
SO  - Dev Neurobiol. 2007 Sep 15;67(11):1488-97. doi: 10.1002/dneu.20523.