PMID- 17530665
OWN - NLM
STAT- MEDLINE
DCOM- 20080306
LR  - 20091005
IS  - 1932-5223 (Print)
IS  - 1932-5223 (Linking)
VI  - 307
IP  - 7
DP  - 2007 Jul 1
TI  - The novel correlation of carbonic anhydrase II and anion exchanger 1 in gills of 
      the spotted green pufferfish, Tetraodon nigrovirids.
PG  - 411-8
AB  - A novel relationship between branchial carbonic anhydrase II (CAII) and anion 
      exchanger 1 (AE1) was investigated in the euryhaline spotted green pufferfish 
      (Tetraodon nigroviridis). The immunoblots revealed that AE1 was only detected in 
      the membrane fraction of gills while CAII can be probed both in the membrane and 
      cytosol fractions of gills. CAII protein abundance in the membrane fraction is 
      salinity dependent. Immunological detection of the membrane fraction CAII protein 
      in gills showed 3.9-fold higher in the hyposmotic (freshwater) group than the 
      hyperosmotic (seawater;35 per thousand) group. In contrast, there was no change 
      in the protein level of cytosolic CAII between seawater and freshwater groups. 
      The whole-mount immunocytochemical staining demonstrated that both AE1 and CAII 
      were colocalized to the Na(+)/K(+)-ATPase-immunoreactive cells in gill epithelium 
      of the pufferfish. The interaction between CAII and AE1 was further identified by 
      co-immunoprecipitation because AE1 was detected in the immunoprecipitates of CAII 
      and vice versa. Our results showed that in pufferfish gills CAII was not only 
      expressed in the cytosol to produce the substrate for AE1 transport during Cl(-) 
      influx but also associated with the plasma membrane via AE1. Obviously, it is 
      essential for the physiological function of AE1 to interact with CAII in the 
      membrane of gill Na(+)/K(+)-ATPase-immunoreactive cells. To our knowledge, this 
      is the first study to demonstrate the interaction of branchial CAII and AE1 in 
      fish. The novel correlation proposed a new model of Cl(-)/HCO(3) (-) transport in 
      gills of the teleosts.
FAU - Tang, C H
AU  - Tang CH
AD  - Department of Life Sciences, National Chung-Hsing University, Taichung, Taiwan.
FAU - Lee, T H
AU  - Lee TH
LA  - eng
GR  - N01 HD62915/HD/NICHD NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Exp Zool A Ecol Genet Physiol
JT  - Journal of experimental zoology. Part A, Ecological genetics and physiology
JID - 101297745
RN  - 0 (Antiporters)
RN  - 0 (Bicarbonates)
RN  - 0 (Chlorides)
RN  - EC 4.2.1.- (Carbonic Anhydrase II)
SB  - IM
MH  - Animals
MH  - Antiporters/*metabolism
MH  - Bicarbonates/metabolism
MH  - Biological Transport/physiology
MH  - Carbonic Anhydrase II/*metabolism
MH  - Cell Membrane/metabolism
MH  - Chlorides/metabolism
MH  - Cytosol/metabolism
MH  - Gills/*metabolism
MH  - Immunoblotting
MH  - Immunohistochemistry
MH  - Immunoprecipitation
MH  - Tetraodontiformes/*metabolism
EDAT- 2007/05/29 09:00
MHDA- 2008/03/07 09:00
CRDT- 2007/05/29 09:00
PHST- 2007/05/29 09:00 [pubmed]
PHST- 2008/03/07 09:00 [medline]
PHST- 2007/05/29 09:00 [entrez]
AID - 10.1002/jez.391 [doi]
PST - ppublish
SO  - J Exp Zool A Ecol Genet Physiol. 2007 Jul 1;307(7):411-8. doi: 10.1002/jez.391.