PMID- 17531990
OWN - NLM
STAT- MEDLINE
DCOM- 20080124
LR  - 20081121
IS  - 1556-5653 (Electronic)
IS  - 0015-0282 (Linking)
VI  - 89
IP  - 1
DP  - 2008 Jan
TI  - Decreased human leukocyte antigen-DR expression in the lipid raft by peritoneal 
      macrophages from women with endometriosis.
PG  - 52-9
AB  - OBJECTIVE: To investigate the macrophage response in endometriosis by determining 
      the expression and localization of human leukocyte antigen (HLA)-ABC and HLA-DR 
      by the peritoneal fluid (PF) macrophages and PF concentrations of interferon 
      (IFN)-gamma that regulate HLA expression. DESIGN: Case-control study. SETTING: 
      University hospital. PATIENT(S): 64 Japanese endometriosis patients, and 65 women 
      with other laparoscopic diagnoses. INTERVENTION(S): Venipuncture and laparoscopic 
      peritoneal fluid collection. MAIN OUTCOME MEASURE(S): Expression and localization 
      of HLA-ABC and HLA-DR in PF macrophages were determined by flow cytometry and 
      confocal microscopy. The concentration of IFN-gamma in PF was determined by 
      enzyme-linked immunosorbent assay. RESULT(S): In women with endometriosis, 
      expression of HLA-ABC and HLA-DR by PF macrophages, and the IFN-gamma 
      concentrations in PF were statistically significantly lower than in controls. 
      Women with endometriosis showed a statistically significant positive correlation 
      between HLA expression and IFN-gamma concentration. By confocal microscopy, 
      HLA-ABC was distributed homogenously on the macrophage surface whereas HLA-DR 
      expression on these cells corresponded to the lipid raft. CONCLUSION(S): In women 
      with endometriosis, low HLA expression and particularly reduced HLA-DR in the 
      lipid raft may be influenced by low IFN-gamma and may compromise antigen 
      presentation, limiting the immune response to peritoneal cavity antigens such as 
      implanted or metaplastic endometrial tissue.
FAU - Yamamoto, Yorito
AU  - Yamamoto Y
AD  - Department of Obstetrics and Gynecology, Kochi Medical School, Kohasu, Oko, 
      Nankoku, Kochi, Japan. yoritoy@med.kochi-u.ac.jp
FAU - Maeda, Nagamasa
AU  - Maeda N
FAU - Izumiya, Chiaki
AU  - Izumiya C
FAU - Kusume, Tomoaki
AU  - Kusume T
FAU - Oguri, Hiroyoshi
AU  - Oguri H
FAU - Kawashima, Masaaki
AU  - Kawashima M
FAU - Hayashi, Kazutoshi
AU  - Hayashi K
FAU - Nomura, Aki
AU  - Nomura A
FAU - Yamashita, Chika
AU  - Yamashita C
FAU - Fukaya, Takao
AU  - Fukaya T
LA  - eng
PT  - Journal Article
DEP - 20070525
PL  - United States
TA  - Fertil Steril
JT  - Fertility and sterility
JID - 0372772
RN  - 0 (HLA-DR Antigens)
RN  - 0 (Histocompatibility Antigens Class I)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Adult
MH  - Antigen Presentation
MH  - Ascitic Fluid/*immunology
MH  - Case-Control Studies
MH  - Down-Regulation
MH  - Endometriosis/*immunology
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Female
MH  - Flow Cytometry
MH  - HLA-DR Antigens/*analysis
MH  - Histocompatibility Antigens Class I/*analysis
MH  - Humans
MH  - Interferon-gamma/*analysis
MH  - Macrophages, Peritoneal/*immunology
MH  - Membrane Microdomains/*immunology
MH  - Microscopy, Confocal
MH  - Severity of Illness Index
EDAT- 2007/05/29 09:00
MHDA- 2008/01/25 09:00
CRDT- 2007/05/29 09:00
PHST- 2006/06/06 00:00 [received]
PHST- 2007/02/13 00:00 [revised]
PHST- 2007/02/13 00:00 [accepted]
PHST- 2007/05/29 09:00 [pubmed]
PHST- 2008/01/25 09:00 [medline]
PHST- 2007/05/29 09:00 [entrez]
AID - S0015-0282(07)00448-7 [pii]
AID - 10.1016/j.fertnstert.2007.02.027 [doi]
PST - ppublish
SO  - Fertil Steril. 2008 Jan;89(1):52-9. doi: 10.1016/j.fertnstert.2007.02.027. Epub 
      2007 May 25.