PMID- 17532793
OWN - NLM
STAT- MEDLINE
DCOM- 20070731
LR  - 20231213
IS  - 0953-8194 (Print)
IS  - 0953-8194 (Linking)
VI  - 19
IP  - 7
DP  - 2007 Jul
TI  - Identification of central nervous system sites involved in the water diuresis 
      response elicited by central microinjection of nociceptin/ Orphanin FQ in 
      conscious rats via c-Fos and inducible cAMP early repressor immunocytochemistry.
PG  - 531-42
AB  - Intracerebroventricular (i.c.v.) administration of the opioid-like peptide, 
      nociceptin/Orphanin (nociceptin), in conscious rats produces diuretic and 
      antinatriuretic effects. The present study utilised changes in Fos and inducible 
      cAMP early repressor (ICER) immunocytochemistry expression to examine the central 
      nervous (CNS) sites activated or inhibited, respectively, by central 
      administration of nociceptin. Urine samples were collected during control (15 
      min) and after i.c.v. vehicle (5 microl, n = 12) or nociceptin (10 microg/5 
      microl; n = 12). Four additional urine samples (15-min) were collected after the 
      i.c.v. injection. The brain was processed for Fos using a commercially available 
      antibody (Oncogene AB-5) and for ICER using a polyclonal anti-ICER antibody 
      raised in rabbits. In vehicle-injected conscious rats, renal excretion of water 
      or sodium was not altered. However, nociceptin produced a rapid and marked 
      increase in urine flow (V) and a decrease in urinary sodium excretion rate. In 
      addition, i.c.v. nociceptin produced a significant increase in Fos staining in 
      the dorsomedial nucleus of the hypothalamus, the perinuclear zone of the 
      supraoptic nucleus, the organum vasculosum of the lamina terminalis (OVLT), the 
      lateral preoptic area and the lateral hypothalamic area compared to control. By 
      contrast, Fos expression decreased in the area postrema and locus coeruleus 
      compared to controls. Furthermore, ICER staining was significantly increased in 
      the perinuclear zone of the supraoptic nucleus, supraoptic nucleus, median 
      preoptic nucleus, OVLT, medial preoptic area, central nucleus of the amygdala, 
      and medial nucleus of the solitary tract. Together, central opioid receptor-like 
      type 1 activation in these CNS regions may participate in the neural pathways 
      involved in the diuretic and antinatriuretic effects of nociceptin.
FAU - Gottlieb, H B
AU  - Gottlieb HB
AD  - Department of Pharmacology, University of Texas Health Science Center, San 
      Antonio, TX 78229, USA.
FAU - Fleming, T M
AU  - Fleming TM
FAU - Ji, L
AU  - Ji L
FAU - Cunningham, J T
AU  - Cunningham JT
LA  - eng
GR  - R01 HL062579/HL/NHLBI NIH HHS/United States
GR  - R01 DK57822/DK/NIDDK NIH HHS/United States
GR  - R01 HL55692/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PL  - United States
TA  - J Neuroendocrinol
JT  - Journal of neuroendocrinology
JID - 8913461
RN  - 0 (Opioid Peptides)
RN  - 0 (Proto-Oncogene Proteins c-fos)
RN  - 0 (Repressor Proteins)
RN  - E0399OZS9N (Cyclic AMP)
SB  - IM
MH  - Animals
MH  - Central Nervous System/*drug effects/metabolism
MH  - Cyclic AMP/*metabolism
MH  - *Diuresis
MH  - Immunohistochemistry
MH  - Microinjections
MH  - Opioid Peptides/*administration & dosage
MH  - Proto-Oncogene Proteins c-fos/*metabolism
MH  - Rats
MH  - Repressor Proteins/*metabolism
MH  - Nociceptin
EDAT- 2007/05/30 09:00
MHDA- 2007/08/01 09:00
CRDT- 2007/05/30 09:00
PHST- 2007/05/30 09:00 [pubmed]
PHST- 2007/08/01 09:00 [medline]
PHST- 2007/05/30 09:00 [entrez]
AID - JNE1559 [pii]
AID - 10.1111/j.1365-2826.2007.01559.x [doi]
PST - ppublish
SO  - J Neuroendocrinol. 2007 Jul;19(7):531-42. doi: 10.1111/j.1365-2826.2007.01559.x.