PMID- 17533049
OWN - NLM
STAT- MEDLINE
DCOM- 20070716
LR  - 20181201
IS  - 0301-472X (Print)
IS  - 0301-472X (Linking)
VI  - 35
IP  - 6
DP  - 2007 Jun
TI  - Adiponectin binds to chemokines via the globular head and modulates interactions 
      between chemokines and heparan sulfates.
PG  - 947-56
AB  - OBJECTIVE: Adiponectin, a fat cell-derived protein, has been attracting 
      considerable attention because of its antidiabetic and antiatherogenic 
      activities. The aim of the present study is to identify molecules physiologically 
      associating with adiponectin and to understand how the protein displays diverse 
      biological activities. MATERIALS AND METHODS: We used an expression cloning 
      method combined with enzyme-linked immunosorbent assay to clone 
      adiponectin-binding proteins from the MS-5 complementary DNA library. RESULTS: We 
      successfully isolated two chemokines, stromal cell-derived factor-1 (SDF-1) and 
      CCF18, and verified that adiponectin bound to them via its globular head. 
      Adiponectin bound with various chemokines in vitro, such as 
      macrophage-inflammatory protein-1alpha (MIP-1alpha), RANTES, and monocyte 
      chemoattractant protein-1 (MCP-1), suggesting that the protein had a feature 
      commonly to bind to the chemokine family. The middle part of chemokines, 
      dispensable for interacting with their receptors, was found to be important for 
      the adiponectin binding. Although the interaction of adiponectin to SDF-1 
      affected neither the SDF-1-CXCR4 binding nor the SDF-1 signaling in Jurkat cells, 
      adiponectin and heparin mutually interfered in their association to SDF-1 and 
      MCP-1 in vitro, implying that their association might influence the distribution 
      of adiponectin and SDF-1 in inflammatory sites. Indeed, both adiponectin and 
      SDF-1 was positively immunostained in vascular walls in guts from acute 
      graft-vs-host disease patients. In addition, peripheral blood of 
      adiponectin-deficient mice contained more hematopoietic progenitors than that of 
      wild-type mice. CONCLUSION: Adiponectin may be involved in regulation of 
      inflammation via binding to specific chemokines. Additionally, the interaction 
      possibly enables adiponectin to gather and play its role in inflammatory sites.
FAU - Masaie, Hiroaki
AU  - Masaie H
AD  - Department of Hematology and Oncology, Osaka University Graduate School of 
      Medicine, Osaka, Japan.
FAU - Oritani, Kenji
AU  - Oritani K
FAU - Yokota, Takafumi
AU  - Yokota T
FAU - Takahashi, Isao
AU  - Takahashi I
FAU - Shirogane, Takahiro
AU  - Shirogane T
FAU - Ujiie, Hidetoshi
AU  - Ujiie H
FAU - Ichii, Michiko
AU  - Ichii M
FAU - Saitoh, Norimitsu
AU  - Saitoh N
FAU - Maeda, Tetsuo
AU  - Maeda T
FAU - Tanigawa, Rie
AU  - Tanigawa R
FAU - Oka, Kazumasa
AU  - Oka K
FAU - Hoshida, Yoshihiko
AU  - Hoshida Y
FAU - Tomiyama, Yoshiaki
AU  - Tomiyama Y
FAU - Kanakura, Yuzuru
AU  - Kanakura Y
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Exp Hematol
JT  - Experimental hematology
JID - 0402313
RN  - 0 (Adiponectin)
RN  - 0 (Chemokines)
RN  - 9050-30-0 (Heparitin Sulfate)
SB  - IM
MH  - Acute Disease
MH  - Adiponectin/genetics/*metabolism
MH  - Animals
MH  - Chemokines/genetics/*metabolism
MH  - Cloning, Molecular
MH  - Graft vs Host Disease/genetics/metabolism/pathology
MH  - HeLa Cells
MH  - Hematopoietic Stem Cells/metabolism/pathology
MH  - Heparitin Sulfate/genetics/*metabolism
MH  - Humans
MH  - Inflammation/genetics/metabolism/pathology
MH  - Intestinal Diseases/genetics/metabolism/pathology
MH  - Intestinal Mucosa/metabolism
MH  - Intestines/pathology
MH  - Jurkat Cells
MH  - Mice
MH  - Mice, Knockout
MH  - Protein Binding
MH  - Signal Transduction
EDAT- 2007/05/30 09:00
MHDA- 2007/07/17 09:00
CRDT- 2007/05/30 09:00
PHST- 2006/04/12 00:00 [received]
PHST- 2007/03/15 00:00 [revised]
PHST- 2007/03/15 00:00 [accepted]
PHST- 2007/05/30 09:00 [pubmed]
PHST- 2007/07/17 09:00 [medline]
PHST- 2007/05/30 09:00 [entrez]
AID - S0301-472X(07)00193-2 [pii]
AID - 10.1016/j.exphem.2007.03.010 [doi]
PST - ppublish
SO  - Exp Hematol. 2007 Jun;35(6):947-56. doi: 10.1016/j.exphem.2007.03.010.