PMID- 17533151
OWN - NLM
STAT- MEDLINE
DCOM- 20071025
LR  - 20161124
IS  - 1286-4579 (Print)
IS  - 1286-4579 (Linking)
VI  - 9
IP  - 7
DP  - 2007 Jun
TI  - Treponema denticola induces interleukin-8 and macrophage chemoattractant protein 
      1 production in human umbilical vein epithelial cells.
PG  - 907-13
AB  - Treponema denticola, a major pathogen of periodontitis, has also been detected in 
      the lesions of atherosclerosis. The aim of this study was to investigate 
      induction of chemokine production in human umbilical vein endothelial cells 
      (HUVECs) by T. denticola and determine whether those chemokines were degraded by 
      a protease, dentilisin. T. denticola ATCC35405 or dentilisin-deficient mutant K1 
      were added to HUVECs and levels of interleukin-8 (IL-8) and monocyte 
      chemoattractant protein-1 (MCP-1) in the culture supernatants were determined by 
      enzyme-linked immunosorbent assay. T. denticola ATCC35405 induced production of 
      IL-8 in a time-dependent manner, with both production of IL-8 and expression of 
      IL-8 mRNA showing higher levels than with exposure to dentilisin-deficient mutant 
      K1. Although exposure to ATCC35405 induced expression of MCP-1 mRNA in the 
      HUVECs, MCP-1 levels were remained similar to that in unstimulated cells. IL-8 
      and MCP-1 showed partial hydrolysis with exposure to T. denticola ATCC35405, but 
      not with T. denticola K1. These results suggest that T. denticola can evade host 
      defense mechanisms by modulating production of IL-8 and MCP-1, and that this play 
      a role in the development of chronic infections such as periodontitis. The 
      association of T. denticola infection to atherosclerosis was also discussed based 
      on the present study.
FAU - Okuda, Tomoko
AU  - Okuda T
AD  - Department of Periodontology, Tokyo Dental College, 1-2-2 Masago, Mihama-ku, 
      Chiba 261-8502, Japan.
FAU - Kimizuka, Ryuta
AU  - Kimizuka R
FAU - Miyamoto, Meguru
AU  - Miyamoto M
FAU - Kato, Tetsuo
AU  - Kato T
FAU - Yamada, Satoru
AU  - Yamada S
FAU - Okuda, Katsuji
AU  - Okuda K
FAU - Ishihara, Kazuyuki
AU  - Ishihara K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20070321
PL  - France
TA  - Microbes Infect
JT  - Microbes and infection
JID - 100883508
RN  - 0 (Bacterial Proteins)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Interleukin-8)
RN  - 63231-63-0 (RNA)
RN  - EC 3.4.- (Peptide Hydrolases)
RN  - EC 3.4.21.- (dentilisin)
RN  - EC 3.4.21.1 (Chymotrypsin)
SB  - IM
MH  - Bacterial Proteins
MH  - Chemokine CCL2/*biosynthesis/genetics/immunology
MH  - Chymotrypsin/metabolism
MH  - Endothelial Cells/immunology/microbiology
MH  - Epithelial Cells/immunology/microbiology
MH  - Humans
MH  - Interleukin-8/*biosynthesis/genetics/immunology
MH  - Peptide Hydrolases
MH  - Periodontitis/*immunology/microbiology
MH  - RNA/chemistry/genetics
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Treponema denticola/enzymology/*immunology
MH  - Umbilical Veins/cytology
EDAT- 2007/05/30 09:00
MHDA- 2007/10/27 09:00
CRDT- 2007/05/30 09:00
PHST- 2006/09/05 00:00 [received]
PHST- 2007/03/14 00:00 [revised]
PHST- 2007/03/15 00:00 [accepted]
PHST- 2007/05/30 09:00 [pubmed]
PHST- 2007/10/27 09:00 [medline]
PHST- 2007/05/30 09:00 [entrez]
AID - S1286-4579(07)00124-4 [pii]
AID - 10.1016/j.micinf.2007.03.009 [doi]
PST - ppublish
SO  - Microbes Infect. 2007 Jun;9(7):907-13. doi: 10.1016/j.micinf.2007.03.009. Epub 
      2007 Mar 21.