PMID- 17534122
OWN - NLM
STAT- MEDLINE
DCOM- 20071004
LR  - 20171116
IS  - 0031-7012 (Print)
IS  - 0031-7012 (Linking)
VI  - 80
IP  - 2-3
DP  - 2007
TI  - Involvement of NMDA receptors in thiopental-induced loss of righting reflex, 
      antinociception and anticonvulsion effects in mice.
PG  - 127-33
AB  - Potentiation of GABA(A) receptor-mediated inhibitory neurotransmission 
      contributes to the anesthetic action of thiopental. However, the inhibiting 
      action of general anesthetic on excitatory neurotransmission also purportedly 
      underlies its effects. The aim of the study was to elucidate the role of 
      glutamate receptors (NMDA and AMPA receptors) in thiopental-induced anesthesia. 
      Intracerebroventricular (i.c.v.) NMDA (50 ng) significantly increased the 
      induction time of loss of righting reflex and decreased sleep time induced by 
      intraperitoneal injection (i.p.) of thiopental (50 mg/kg). Furthermore, NMDA at 
      50 ng i.c.v. increased the 50% effective dose values for thiopental to produce 
      loss of righting reflex and immobility in response to noxious tail clamp by 25% 
      and 21% (p < 0.05), respectively. However, intrathecal (IT) administration of 
      NMDA or both of i.c.v. or IT administration of AMPA did not show such 
      antagonizing effects on thiopental action at subconvulsive dose. Finally, 
      thiopental (25 mg/kg i.p.) inhibited convulsions induced by NMDA (0.4 microg 
      i.c.v.) or bicuculline (0.6 microg i.c.v.). However, i.p. muscimol (1 mg/kg) 
      blocked the convulsions induced by bicuculline, but not those induced by NMDA at 
      3 mg/kg. Similarly, i.p. MK-801 (0.1 mg/kg) antagonized NMDA-induced convulsions, 
      but not bicuculline-induced convulsions at 0.3 mg/kg. Therefore, we suggest that 
      the effects of the selective GABA(A) and NMDA receptors on convulsive behavior 
      are special to their sites of action, and that the inhibitory action of 
      thiopental on NMDA receptors is possibly not mediated by secondary effects of its 
      GABA(A) receptors agonism. These results above indicate the involvement of NMDA 
      receptors in thiopental-induced anesthesia in mice.
CI  - Copyright (c) 2007 S. Karger AG, Basel.
FAU - Ge, Zhi Jun
AU  - Ge ZJ
AD  - Department of Anesthesiology, Affiliated Hospital of First Clinical College, 
      China Medical University, Shenyang, PR China.
FAU - Zhang, Li Cai
AU  - Zhang LC
FAU - Zeng, Yin Ming
AU  - Zeng YM
FAU - Da, Ti Jun
AU  - Da TJ
FAU - Wang, Jun Ke
AU  - Wang JK
FAU - Cui, Guo Xin
AU  - Cui GX
FAU - Tan, Yong Fei
AU  - Tan YF
FAU - Zhao, Yan Ping
AU  - Zhao YP
FAU - Liu, Guo Jun
AU  - Liu GJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20070529
PL  - Switzerland
TA  - Pharmacology
JT  - Pharmacology
JID - 0152016
RN  - 0 (Analgesics)
RN  - 0 (Anesthetics, Intravenous)
RN  - 0 (Anticonvulsants)
RN  - 0 (GABA Agonists)
RN  - 0 (GABA Antagonists)
RN  - 0 (GABA-A Receptor Agonists)
RN  - 0 (GABA-A Receptor Antagonists)
RN  - 0 (Receptors, AMPA)
RN  - 0 (Receptors, N-Methyl-D-Aspartate)
RN  - 2763-96-4 (Muscimol)
RN  - 6384-92-5 (N-Methylaspartate)
RN  - 6LR8C1B66Q (Dizocilpine Maleate)
RN  - JI8Z5M7NA3 (Thiopental)
RN  - Y37615DVKC (Bicuculline)
SB  - IM
MH  - Analgesics/*pharmacology
MH  - Anesthetics, Intravenous/*pharmacology
MH  - Animals
MH  - Anticonvulsants/*pharmacology
MH  - Behavior, Animal/drug effects
MH  - Bicuculline
MH  - Dizocilpine Maleate/pharmacology
MH  - Female
MH  - GABA Agonists/pharmacology
MH  - GABA Antagonists/pharmacology
MH  - GABA-A Receptor Agonists
MH  - GABA-A Receptor Antagonists
MH  - Male
MH  - Mice
MH  - Movement/drug effects
MH  - Muscimol/pharmacology
MH  - N-Methylaspartate
MH  - Pain Measurement
MH  - Receptors, AMPA/agonists/antagonists & inhibitors/physiology
MH  - Receptors, N-Methyl-D-Aspartate/agonists/antagonists & inhibitors/*physiology
MH  - Reflex, Abnormal/*drug effects
MH  - Seizures/chemically induced/physiopathology/prevention & control
MH  - Thiopental/*pharmacology
EDAT- 2007/05/31 09:00
MHDA- 2007/10/05 09:00
CRDT- 2007/05/31 09:00
PHST- 2006/06/12 00:00 [received]
PHST- 2007/01/11 00:00 [accepted]
PHST- 2007/05/31 09:00 [pubmed]
PHST- 2007/10/05 09:00 [medline]
PHST- 2007/05/31 09:00 [entrez]
AID - 000103252 [pii]
AID - 10.1159/000103252 [doi]
PST - ppublish
SO  - Pharmacology. 2007;80(2-3):127-33. doi: 10.1159/000103252. Epub 2007 May 29.