PMID- 17535564
OWN - NLM
STAT- MEDLINE
DCOM- 20071206
LR  - 20151119
IS  - 0161-6412 (Print)
IS  - 0161-6412 (Linking)
VI  - 29
IP  - 5
DP  - 2007 Jul
TI  - The changes in neurotrophic properties of the peripheral nerves extracts 
      following blocking of BDNF activity.
PG  - 500-5
AB  - OBJECTIVES: Retinal ganglion cells (RGCs) of adult rats are unable to regenerate 
      their axons after optic nerve injury and soon after they enter the pathway of 
      apoptosis. They may, however, survive and regenerate new axons in response to 
      application of specific peripheral nerve extracts that presumably contain a range 
      of neurotrophic substances. One of the recognized substances of proven 
      neurotrophic activity is brain-derived neurotrophic factor (BDNF). We have 
      investigated whether blocking the BDNF activity in post-microsomal fractions 
      obtained from 7 day pre-degenerated peripheral nerves would affect its 
      neurotrophic properties towards RGCs after optic nerve transection in adult rats. 
      METHODS: Autologous connective tissue chambers sutured to the distal end of 
      transected optic nerve served as active substances containers. Surviving RGCs 
      were visualized using Dil. The number of myelinated outgrowing fibers within the 
      chambers was evaluated in histologic sections. RESULTS: BDNF and 7 day 
      pre-degenerated nerve extracts, and also extracts with blocked BDNF activity, 
      enhanced RGC fibers outgrowth. The regeneration was significantly weaker in the 
      control group. Blocking the BDNF activity in the 7 day pre-degenerated peripheral 
      nerve extract reduced its neurotrophic effects but the differences were 
      insignificant in comparison with non-blocked extracts. DISCUSSION: The 
      regeneration intensities in groups receiving 7 day pre-degenerated peripheral 
      nerve extracts (PD7) and BDNF were comparable. The number of surviving cells was 
      higher in the PD7 group and there were more regenerating fibers in the BDNF 
      group, which may be explained by the strong BDNF effect on axonal 
      collateralization and sprouting.
FAU - Golka, Beata
AU  - Golka B
AD  - Department of Physiology, Medical University of Silesia, ul. Medykow 16, 40-752 
      Katowice, Poland. dargolka@wp.pl
FAU - Swiech-Sabuda, Ewa
AU  - Swiech-Sabuda E
FAU - Golka, Dariusz
AU  - Golka D
FAU - Marcol, Wieslaw
AU  - Marcol W
FAU - Gorka, Dariusz
AU  - Gorka D
FAU - Pietrucha-Dutczak, Marita
AU  - Pietrucha-Dutczak M
FAU - Lewin-Kowalik, Joanna
AU  - Lewin-Kowalik J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Neurol Res
JT  - Neurological research
JID - 7905298
RN  - 0 (Amino Acids)
RN  - 0 (Antibodies)
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (dolaisoleucine)
SB  - IM
MH  - Amino Acids
MH  - Analysis of Variance
MH  - Animals
MH  - Antibodies/pharmacology
MH  - Brain-Derived Neurotrophic Factor/immunology/*physiology
MH  - Cell Count
MH  - In Vitro Techniques
MH  - Microscopy, Electron, Transmission
MH  - Nerve Fibers, Myelinated/drug effects/metabolism/pathology/ultrastructure
MH  - Optic Nerve Injuries/*metabolism/*physiopathology
MH  - Peripheral Nerves/*metabolism/pathology
MH  - Rats
MH  - Rats, Wistar
MH  - Retinal Ganglion Cells/drug effects/*physiology
MH  - Time Factors
EDAT- 2007/05/31 09:00
MHDA- 2007/12/07 09:00
CRDT- 2007/05/31 09:00
PHST- 2007/05/31 09:00 [pubmed]
PHST- 2007/12/07 09:00 [medline]
PHST- 2007/05/31 09:00 [entrez]
AID - 10.1179/016164107X164111 [doi]
PST - ppublish
SO  - Neurol Res. 2007 Jul;29(5):500-5. doi: 10.1179/016164107X164111.