PMID- 17536825
OWN - NLM
STAT- MEDLINE
DCOM- 20070813
LR  - 20131121
IS  - 0021-8561 (Print)
IS  - 0021-8561 (Linking)
VI  - 55
IP  - 13
DP  - 2007 Jun 27
TI  - Development of an analytical method for the determination of beta2-agonist 
      residues in animal tissues by high-performance liquid chromatography with on-line 
      electrogenerated [Cu(HIO6)2]5- -luminol chemiluminescence detection.
PG  - 4949-56
AB  - A novel method was developed for the simultaneous determination of beta2-agonist 
      residues such as terbutaline, salbutamol, and clenbuterol by high-performance 
      liquid chromatography (HPLC) coupled with chemiluminescence (CL) detection. The 
      procedure was based on the enhancement effect of beta2-agonists on the CL 
      reaction between luminol and the complex of trivalent copper and periodate 
      ([Cu(HIO6)2]5-), which was on-line electrogenerated by constant current 
      electrolysis. The HPLC separation used a Nucleosil RP-C18 column (250 mm x 4.6 mm 
      i.d., 5 microm; pore size, 100 A) with a mobile phase consisting of 90% 
      acetonitrile and 10% aqueous ammonium acetate (20 mmol L-1, pH 4.0) at a flow 
      rate of 1.0 mL min-1. The effects of several parameters on the HPLC resolution 
      and CL emission were studied systematically. Liver samples were hydrolyzed with 
      beta-glucuronidase followed by a solid-phase extraction procedure using Waters 
      OasisMCX cartridges. Under optimum conditions, the limits of detection at a 
      signal-to-noise ratio of 3 ranged from 0.007 to 0.01 ng g-1 and the limits of 
      quantification at a signal-to-noise ratio of 10 ranged from 0.023 to 0.033 ng g-1 
      for three beta2-agonists. The relative standard deviations (RSDs) of intra- and 
      interday precision were below 4.5%. The average recoveries for beta2-agonists 
      (spiked at the levels of 0.05-5.0 ng g-1) in pig liver ranged from 84 to 110%, 
      and the RSDs of the quantitative results were from 1.6 to 7.2%. The proposed 
      method was successfully applied to the determination of beta2-agonist residues in 
      pig liver samples.
FAU - Zhang, Yantu
AU  - Zhang Y
AD  - College of Chemistry and Materials Science, Shaanxi Normal University, Xi'an 
      710062, China.
FAU - Zhang, Zhujun
AU  - Zhang Z
FAU - Sun, Yonghua
AU  - Sun Y
FAU - Wei, Yue
AU  - Wei Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20070531
PL  - United States
TA  - J Agric Food Chem
JT  - Journal of agricultural and food chemistry
JID - 0374755
RN  - 0 (Adrenergic beta-Agonists)
RN  - 10450-60-9 (Periodic Acid)
RN  - 789U1901C5 (Copper)
SB  - IM
MH  - Adrenergic beta-Agonists/*analysis
MH  - Animals
MH  - Chromatography, High Pressure Liquid/*methods
MH  - *Copper
MH  - Drug Residues/analysis
MH  - Electrochemistry
MH  - Food Contamination/*analysis
MH  - Liver/chemistry
MH  - Luminescent Measurements
MH  - Meat/analysis
MH  - *Periodic Acid
MH  - Swine
EDAT- 2007/06/01 09:00
MHDA- 2007/08/19 09:00
CRDT- 2007/06/01 09:00
PHST- 2007/06/01 09:00 [pubmed]
PHST- 2007/08/19 09:00 [medline]
PHST- 2007/06/01 09:00 [entrez]
AID - 10.1021/jf070144y [doi]
PST - ppublish
SO  - J Agric Food Chem. 2007 Jun 27;55(13):4949-56. doi: 10.1021/jf070144y. Epub 2007 
      May 31.