PMID- 17538966
OWN - NLM
STAT- MEDLINE
DCOM- 20070712
LR  - 20191210
IS  - 0270-9139 (Print)
IS  - 0270-9139 (Linking)
VI  - 45
IP  - 6
DP  - 2007 Jun
TI  - Remarkable heterogeneity displayed by oval cells in rat and mouse models of stem 
      cell-mediated liver regeneration.
PG  - 1462-70
AB  - The experimental protocols used in the investigation of stem cell-mediated liver 
      regeneration in rodents are characterized by activation of the hepatic stem cell 
      compartment in the canals of Hering followed by transit amplification of oval 
      cells and their subsequent differentiation along hepatic lineages. Although the 
      protocols are numerous and often used interchangeably across species, a thorough 
      comparative phenotypic analysis of oval cells in rats and mice using 
      well-established and generally acknowledged molecular markers has not been 
      provided. In the present study, we evaluated and compared the molecular 
      phenotypes of oval cells in several of the most commonly used protocols of stem 
      cell-mediated liver regeneration-namely, treatment with 2-acetylaminofluorene and 
      partial (70%) hepatectomy (AAF/PHx); a choline-deficient, ethionine-supplemented 
      (CDE) diet; a 3,5-diethoxycarbonyl-1,4-dihydro-collidin (DDC) diet; and 
      N-acetyl-paraaminophen (APAP). Reproducibly, oval cells showing reactivity for 
      cytokeratins (CKs), muscle pyruvate kinase (MPK), the adenosine 
      triphosphate-binding cassette transporter ABCG2/BCRP1 (ABCG2), alpha-fetoprotein 
      (AFP), and delta-like protein 1/preadipocyte factor 1 (Dlk/Pref-1) were induced 
      in rat liver treated according to the AAF/PHx and CDE but not the DDC protocol. 
      In mouse liver, the CDE, DDC, and APAP protocols all induced CKs and 
      ABCG2-positive oval cells. However, AFP and Dlk/Pref-1 expression was rarely 
      detected in oval cells. CONCLUSION: Our results delineate remarkable phenotypic 
      discrepancies exhibited by oval cells in stem cell-mediated liver regeneration 
      between rats and mice and underline the importance of careful extrapolation 
      between individual species.
FAU - Jelnes, Peter
AU  - Jelnes P
AD  - Danish Stem Cell Research Centre, Department of Cellular and Molecular Medicine, 
      The Panum Institute, University of Copenhagen, Blegdamsvej 3, DK-2200 Copenhagen, 
      Denmark.
FAU - Santoni-Rugiu, Eric
AU  - Santoni-Rugiu E
FAU - Rasmussen, Morten
AU  - Rasmussen M
FAU - Friis, Susanne Lunoe
AU  - Friis SL
FAU - Nielsen, Jens Hoiriis
AU  - Nielsen JH
FAU - Tygstrup, Niels
AU  - Tygstrup N
FAU - Bisgaard, Hanne Cathrine
AU  - Bisgaard HC
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Hepatology
JT  - Hepatology (Baltimore, Md.)
JID - 8302946
RN  - 0 (ATP Binding Cassette Transporter, Subfamily G, Member 2)
RN  - 0 (ATP-Binding Cassette Transporters)
RN  - 0 (Abcg2 protein, mouse)
RN  - 0 (Abcg2 protein, rat)
RN  - 0 (Biomarkers)
RN  - 0 (Calcium-Binding Proteins)
RN  - 0 (Dlk1 protein, mouse)
RN  - 0 (Dlk1 protein, rat)
RN  - 0 (Intercellular Signaling Peptides and Proteins)
RN  - 0 (Membrane Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (alpha-Fetoproteins)
RN  - 68238-35-7 (Keratins)
RN  - EC 2.7.1.40 (Pyruvate Kinase)
SB  - IM
MH  - ATP Binding Cassette Transporter, Subfamily G, Member 2
MH  - ATP-Binding Cassette Transporters/genetics/metabolism
MH  - Animals
MH  - Biomarkers/*metabolism
MH  - Calcium-Binding Proteins
MH  - Cell Lineage/physiology
MH  - Female
MH  - Immunohistochemistry
MH  - Intercellular Signaling Peptides and Proteins/genetics/metabolism
MH  - Keratins/metabolism
MH  - Liver/*cytology/*physiology
MH  - Liver Regeneration/*physiology
MH  - Male
MH  - Membrane Proteins/genetics/metabolism
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Models, Animal
MH  - Phenotype
MH  - Pyruvate Kinase/genetics/metabolism
MH  - RNA, Messenger/metabolism
MH  - Rats
MH  - Rats, Inbred F344
MH  - Stem Cells/cytology/*physiology
MH  - alpha-Fetoproteins/genetics/metabolism
EDAT- 2007/06/01 09:00
MHDA- 2007/07/13 09:00
CRDT- 2007/06/01 09:00
PHST- 2007/06/01 09:00 [pubmed]
PHST- 2007/07/13 09:00 [medline]
PHST- 2007/06/01 09:00 [entrez]
AID - 10.1002/hep.21569 [doi]
PST - ppublish
SO  - Hepatology. 2007 Jun;45(6):1462-70. doi: 10.1002/hep.21569.