PMID- 17539252
OWN - NLM
STAT- MEDLINE
DCOM- 20070815
LR  - 20141120
IS  - 0125-1562 (Print)
IS  - 0125-1562 (Linking)
VI  - 38
IP  - 1
DP  - 2007 Jan
TI  - Patient and sample-related factors that effect the success of in vitro isolation 
      of Orientia tsutsugamushi.
PG  - 91-6
AB  - Orientia tsutsugamushi is the causative agent of scrub typhus infection, a major 
      cause of human disease in rural areas of Southeast Asia. Twenty-six blood samples 
      collected from patients with serologically proven scrub typhus during a six month 
      period were sent to Bangkok (535 km from the clinical site) by road at ambient 
      temperature (average daily temperature range: 27.1-29.1 degrees C) for attempted 
      in vitro isolation in Vero cells. O. tsutsugamushi was isolated from 12 samples 
      (sensitivity 46.7%) with the time to isolation ranging from 16 to 37 days [median 
      27 days, inter-quartile range (IQR) 22.5-33.5 days]. Patient factors such as days 
      of fever and O. tsutsugamushi IgM antibody titer, transport factors such as 
      transit time, and isolate genotype (Karp and Gilliam/Kawasaki) were assessed to 
      determine their influence on the outcome of in vitro isolation. None of the 
      factors significantly influenced the isolation outcome. This study demonstrates 
      that O. tsutsugamushi can often be isolated in vitro from the blood of scrub 
      typhus patients when transported at ambient tropical temperatures for many days.
FAU - Luksameetanasan, Rungnapa
AU  - Luksameetanasan R
AD  - Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.
FAU - Blacksell, Stuart D
AU  - Blacksell SD
FAU - Kalambaheti, Thareerat
AU  - Kalambaheti T
FAU - Wuthiekanun, Vanaporn
AU  - Wuthiekanun V
FAU - Chierakul, Wirongrong
AU  - Chierakul W
FAU - Chueasuwanchai, Sunee
AU  - Chueasuwanchai S
FAU - Apiwattanaporn, Apichat
AU  - Apiwattanaporn A
FAU - Stenos, John
AU  - Stenos J
FAU - Graves, Stephen
AU  - Graves S
FAU - Peacock, Sharon J
AU  - Peacock SJ
FAU - Day, Nicholas P J
AU  - Day NP
LA  - eng
GR  - Wellcome Trust/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Thailand
TA  - Southeast Asian J Trop Med Public Health
JT  - The Southeast Asian journal of tropical medicine and public health
JID - 0266303
RN  - 0 (Shiga Toxins)
SB  - IM
MH  - Animals
MH  - Humans
MH  - In Vitro Techniques
MH  - Orientia tsutsugamushi/*isolation & purification
MH  - Rural Population
MH  - Scrub Typhus/*blood/diagnosis/microbiology
MH  - Shiga Toxins/blood
MH  - Specimen Handling/*methods
MH  - Temperature
MH  - Thailand/epidemiology
MH  - Time Factors
EDAT- 2007/06/02 09:00
MHDA- 2007/08/19 09:00
CRDT- 2007/06/02 09:00
PHST- 2007/06/02 09:00 [pubmed]
PHST- 2007/08/19 09:00 [medline]
PHST- 2007/06/02 09:00 [entrez]
PST - ppublish
SO  - Southeast Asian J Trop Med Public Health. 2007 Jan;38(1):91-6.