PMID- 17540495
OWN - NLM
STAT- MEDLINE
DCOM- 20071206
LR  - 20071001
IS  - 0301-2115 (Print)
IS  - 0301-2115 (Linking)
VI  - 134
IP  - 2
DP  - 2007 Oct
TI  - How safe is germinal vesicle stage oocyte rescue? Aneuploidy analysis of in vitro 
      matured oocytes.
PG  - 213-9
AB  - OBJECTIVE: To evaluate the rate and type of aneuploidies of chromosomes 13, 16, 
      18, 21 and 22, with respect to the length of in vitro maturation (IVM) period, 
      and to compare the results to previously published studies on aneuploidy rates of 
      unfertilized, uninseminated mature oocytes and first polar bodies. STUDY DESIGN: 
      Two hundred and twelve immature germinal vesicle stage oocytes were assigned to 
      two groups. After successful IVM, depending on their maturational period of 24h 
      (Group A) or 36h (Group B), chromosomal analysis was performed by five color 
      fluorescence in situ hybridization (FISH). In Groups A and B the rates of 
      aneuploid oocytes were calculated and compared by chi-square test. Also the rates 
      of hyperhaploidy, hypohaploidy, disomy and nullisomy were determined and compared 
      by chi-square test. The difference was considered statistically significant at 
      p-value of <0.05. RESULTS: The prolonged IVM did not significantly affect the 
      aneuploidy rate compared to the shorter maturation period (48.1% and 45.0%, 
      respectively). Regarding the unbalanced premature chromatid separation, no 
      statistically significant difference was found between hyperhaploidy and 
      hypohaploidy (14.8% versus 8.3%). For chromosome nondisjunction, higher frequency 
      of disomy than nullisomy was observed (30.6% versus 14.8%; p<0.05). The estimated 
      global aneuploidy rate was between 42% and 63%. CONCLUSIONS: The aneuploidy rate 
      of IVM GV-oocytes is comparable to the aneuploidy rate of in vivo matured oocytes 
      and first polar bodies, regardless of the length of maturation period. This 
      suggests that the immature oocytes can be used in infertility treatment after 
      they complete maturation.
FAU - Vlaisavljevic, Veljko
AU  - Vlaisavljevic V
AD  - Department of Reproductive Medicine and Gynecologic Endocrinology, Maribor 
      Teaching Hospital, Ljubljanska 5, SI-2000 Maribor, Slovenia.
FAU - Krizancic Bombek, Lidija
AU  - Krizancic Bombek L
FAU - Vokac, Nadja Kokalj
AU  - Vokac NK
FAU - Kovacic, Borut
AU  - Kovacic B
FAU - Cizek-Sajko, Mojca
AU  - Cizek-Sajko M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20070530
PL  - Ireland
TA  - Eur J Obstet Gynecol Reprod Biol
JT  - European journal of obstetrics, gynecology, and reproductive biology
JID - 0375672
SB  - IM
MH  - Adult
MH  - *Aneuploidy
MH  - Chromosomes, Human/*genetics
MH  - Female
MH  - Fertilization in Vitro/*methods
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Infertility, Female/therapy
MH  - Oocyte Retrieval/methods
MH  - Oocytes/*growth & development
MH  - Ovulation Induction/methods
EDAT- 2007/06/02 09:00
MHDA- 2007/12/07 09:00
CRDT- 2007/06/02 09:00
PHST- 2006/08/16 00:00 [received]
PHST- 2007/01/24 00:00 [revised]
PHST- 2007/03/16 00:00 [accepted]
PHST- 2007/06/02 09:00 [pubmed]
PHST- 2007/12/07 09:00 [medline]
PHST- 2007/06/02 09:00 [entrez]
AID - S0301-2115(07)00140-6 [pii]
AID - 10.1016/j.ejogrb.2007.03.013 [doi]
PST - ppublish
SO  - Eur J Obstet Gynecol Reprod Biol. 2007 Oct;134(2):213-9. doi: 
      10.1016/j.ejogrb.2007.03.013. Epub 2007 May 30.