PMID- 17540620
OWN - NLM
STAT- MEDLINE
DCOM- 20070810
LR  - 20131121
IS  - 1521-6616 (Print)
IS  - 1521-6616 (Linking)
VI  - 124
IP  - 1
DP  - 2007 Jul
TI  - Immunogenicity of singlet oxygen modified human DNA: implications for anti-DNA 
      antibodies in systemic lupus erythematosus.
PG  - 83-9
AB  - Reactive oxygen species (ROS)-modified DNA has been shown to be a better and more 
      discriminating immunogen than native DNA (nDNA) for the production of anti-DNA 
      autoantibodies in SLE (systemic lupus erythematosus). Among ROS, the role of 
      hydroxyl radical (.OH) in the induction of damage and modification of nDNA has 
      been extensively studied while such documentation implicating singlet oxygen 
      ((1)O(2)) in inducing immunogenicity in nDNA leading to the production of 
      anti-double-stranded (ds) DNA autoantibodies in SLE is not yet available. This 
      prospective study was undertaken to evaluate the immunogenicity of healthy human 
      dsDNA modified with (1)O(2) generated by methylene blue plus radiant light. 
      Female rabbits were immunized with (1)O(2)-modified human dsDNA to raise 
      anti-dsDNA antibodies. (1)O(2)-modified anti-dsDNA rabbit immune sera and the 
      (1)O(2)-modified anti-dsDNA rabbit purified immunoglobulin G (IgG) were tested 
      against a variety of dsDNA antigenic substrates through direct enzyme-linked 
      immunosorbent assay (ELISA). The immunogenicity of (1)O(2)-modified human dsDNA 
      was further evaluated by studying its immunoreactivity with SLE patients' sera 
      and SLE patients' purified anti-dsDNA IgG. As compared to healthy human sera, 
      (1)O(2)-modified anti-dsDNA rabbit immune sera as well as the (1)O(2)-modified 
      anti-dsDNA rabbit purified IgG demonstrated a strong affinity towards 
      (1)O(2)-modified human dsDNA(.)(1)O(2)-modified human dsDNA proved to be 
      potentially more immunogenic against SLE patients' whole sera and SLE patients' 
      purified IgG as compared to healthy human sera. Our findings suggest that (1)O(2) 
      may also be inducing immunogenicity in native dsDNA resulting in the production 
      of anti-dsDNA autoantibodies as seen in SLE patients.
FAU - Al Arfaj, Abdurahman Saud
AU  - Al Arfaj AS
AD  - Division of Rheumatology, Department of Medicine, College of Medicine, King Saud 
      University, Riyadh, Saudi Arabia. asarfaj@ksu.edu.sa <asarfaj@ksu.edu.sa>
FAU - Chowdhary, Abdul Rauf
AU  - Chowdhary AR
FAU - Khalil, Najma
AU  - Khalil N
FAU - Ali, Rashid
AU  - Ali R
LA  - eng
PT  - Journal Article
DEP - 20070530
PL  - United States
TA  - Clin Immunol
JT  - Clinical immunology (Orlando, Fla.)
JID - 100883537
RN  - 0 (Antibodies, Antinuclear)
RN  - 0 (Immune Sera)
RN  - 17778-80-2 (Singlet Oxygen)
RN  - 9007-49-2 (DNA)
RN  - T42P99266K (Methylene Blue)
SB  - IM
MH  - Animals
MH  - Antibodies, Antinuclear/blood/genetics/*immunology
MH  - Antibody Formation/immunology
MH  - DNA/blood/*chemistry/genetics/*immunology
MH  - DNA Damage
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Female
MH  - Fluorescent Antibody Technique, Indirect
MH  - Humans
MH  - Immune Sera
MH  - Lupus Erythematosus, Systemic/blood/genetics/*immunology
MH  - Methylene Blue/chemistry/radiation effects
MH  - Rabbits
MH  - Singlet Oxygen/*chemistry/immunology
EDAT- 2007/06/02 09:00
MHDA- 2007/08/11 09:00
CRDT- 2007/06/02 09:00
PHST- 2006/11/21 00:00 [received]
PHST- 2006/11/21 00:00 [revised]
PHST- 2007/03/29 00:00 [accepted]
PHST- 2007/06/02 09:00 [pubmed]
PHST- 2007/08/11 09:00 [medline]
PHST- 2007/06/02 09:00 [entrez]
AID - S1521-6616(07)01154-0 [pii]
AID - 10.1016/j.clim.2007.03.548 [doi]
PST - ppublish
SO  - Clin Immunol. 2007 Jul;124(1):83-9. doi: 10.1016/j.clim.2007.03.548. Epub 2007 
      May 30.