PMID- 17541909
OWN - NLM
STAT- MEDLINE
DCOM- 20071126
LR  - 20191210
IS  - 0867-7077 (Print)
IS  - 0867-7077 (Linking)
VI  - 75
IP  - 1
DP  - 2007
TI  - [The significance of spoligotyping method in epidemiological investigations of 
      tuberculosis].
PG  - 22-31
AB  - INTRODUCTION: The control of tuberculosis (TB) requires methods for rapid 
      detection and tracing sources of infection, so that further transmission can be 
      arrested. Recent developments in molecular biology have resulted in techniques 
      that allow prompt identification and tracking specific strains of M. tuberculosis 
      as they spread through the population. Most of these techniques take advantage of 
      M. tuberculosis DNA polymorphism and are based on various repetitive DNA elements 
      as genetic markers. Each method yields strain-specific genetic profiles 
      (fingerprints). Strains showing identical fingerprints are referred to as 
      clustered and are usually associated with recent transmission, whereas strains 
      whose fingerprints are unique are presumed to represent remote transmission, a 
      reactivation of infection acquired in the distant past. MATERIAL AND METHODS: In 
      recent years, spoligotyping has become one of the most widely used genotyping 
      method for epidemiological studies of TB. Spoligotyping is a PCR-based method 
      allowing to analyze strain-dependent polymorphisms observed in spacer sequences 
      present within the direct repeat (DR) genomic region of M. tuberculosis complex 
      strains. Spoligotyping provides some important advantages over other genotyping 
      techniques. These are simplicity, rapidity, high reproducibility and stability of 
      the results, with the latter being expressed in a simple digital pattern, readily 
      named and databased, and the ability to perform spoligotyping directly on 
      clinical samples, without the need for prior culture. However, spoligotyping has 
      relatively low discriminatory capacity, which makes it necessary to use secondary 
      fingerprinting methods to prove clonality between isolates. RESULTS: The aim of 
      this study was to evaluate the usefulness of spoligotyping in epidemiological 
      investigations of TB by analyzing 16 isoniazid-resistant M. tuberculosis strains 
      isolated from patients with pulmonary TB in the central region of Poland. A total 
      of 11 distinct spoligopatterns were obtained. 9 isolates were represented by a 
      unique pattern, whereas 7 were clustered in 2 groups of 5 and 2 isolates, 
      respectively. When compared with an international spoligodatabase SpolDB4, 13 
      isolates shared already described spoligotypes, whereas 3 did not match any 
      existing spoligopattern in database and were defined as orphans. Spoligotyping 
      overestimated the number of clustered isolates in one of its two clusters when 
      compared to IS6110 Mtb1/ /Mtb2 PCR. Strains clustered using the latter method 
      were assumed to be closely epidemiologically related. CONCLUSION: This report 
      demonstrates the utility of spoligotyping as an initial screening technique, to 
      be supplemented by another typing method of greater discriminatory power, such as 
      the IS6110 Mtb1/Mtb2 PCR in order to better recognize the epidemiological links 
      between TB patients.
FAU - Augustynowicz-Kopec, Ewa
AU  - Augustynowicz-Kopec E
AD  - Zaklad Mikrobiologii Instytutu Gruzlicy i Chorob Pluc w Warszawie. 
      e.kopec@igichp.edu.pl
FAU - Jagielski, Tomasz
AU  - Jagielski T
FAU - Kozinska, Monika
AU  - Kozinska M
FAU - Zabost, Anna
AU  - Zabost A
FAU - Zwolska, Zofia
AU  - Zwolska Z
LA  - pol
PT  - Evaluation Study
PT  - Journal Article
TT  - Znaczenie metody spoligotyping epidemiologicznych dochodzeniach gruzlicy.
PL  - Poland
TA  - Pneumonol Alergol Pol
JT  - Pneumonologia i alergologia polska
JID - 9302892
RN  - 0 (Antitubercular Agents)
RN  - 0 (Genetic Markers)
RN  - V83O1VOZ8L (Isoniazid)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Antitubercular Agents/therapeutic use
MH  - *Bacterial Typing Techniques
MH  - Cluster Analysis
MH  - *Contact Tracing
MH  - DNA Fingerprinting
MH  - Databases, Factual
MH  - Female
MH  - Genetic Markers
MH  - Humans
MH  - Interspersed Repetitive Sequences
MH  - Isoniazid/therapeutic use
MH  - Male
MH  - Middle Aged
MH  - Molecular Epidemiology/*methods
MH  - Mycobacterium tuberculosis/*classification/drug effects/*genetics
MH  - Poland/epidemiology
MH  - Polymerase Chain Reaction
MH  - Polymorphism, Restriction Fragment Length
MH  - Sensitivity and Specificity
MH  - Tuberculosis, Multidrug-Resistant/epidemiology/genetics/microbiology
MH  - Tuberculosis, Pulmonary/*epidemiology/*genetics/microbiology
EDAT- 2007/06/02 09:00
MHDA- 2007/12/06 09:00
CRDT- 2007/06/02 09:00
PHST- 2007/06/02 09:00 [pubmed]
PHST- 2007/12/06 09:00 [medline]
PHST- 2007/06/02 09:00 [entrez]
PST - ppublish
SO  - Pneumonol Alergol Pol. 2007;75(1):22-31.