PMID- 17541942
OWN - NLM
STAT- MEDLINE
DCOM- 20071025
LR  - 20171116
IS  - 0260-437X (Print)
IS  - 0260-437X (Linking)
VI  - 27
IP  - 5
DP  - 2007 Sep-Oct
TI  - B220 analysis with the local lymph node assay: proposal for a more flexible 
      prediction model.
PG  - 506-10
AB  - The mouse local lymph node assay (LLNA) has been developed and validated for the 
      identification of chemicals that have the potential to induce skin sensitisation. 
      In common with other predictive test methods the accuracy of the LLNA is not 
      absolute and experience has revealed that a few chemicals, including for instance 
      a minority of skin irritants, may elicit false-positive reactions in the assay. 
      To improve further the performance of the LLNA, and to eliminate or reduce 
      false-positives, there has been interest in an adjunct method in which the 
      ability of chemicals to cause increases in the frequency of B220(+) lymphocytes 
      in skin-draining lymph nodes is measured. Previous studies suggest that the use 
      of B220 analyses aligned with the standard LLNA may serve to distinguish further 
      between contact allergens and skin irritants. In the original predictive model, 
      chemicals were regarded as being skin sensitisers if they were able to induce a 
      1.25-fold or greater increase in the percentage of B220(+) cells within lymph 
      nodes compared with concurrent vehicle controls. Although this first prediction 
      model has proven useful, in the light of more recent experience, and specifically 
      as a consequence of some variability observed in the frequency of B220(+) 
      lymphocytes in nodes taken from vehicle control-treated animals, it is timely now 
      to reconsider and refine the model. As a result a new prediction model is 
      proposed in which reliance on the use of absolute thresholds is reduced, and in 
      which small changes in control values can be better accommodated.
CI  - (c) 2007 John Wiley & Sons, Ltd.
FAU - Betts, Catherine J
AU  - Betts CJ
AD  - Syngenta Central Toxicology Laboratory, Alderley Park, Macclesfield, Cheshire 
      SK10 4TJ, UK. catherine.betts@syngenta.com
FAU - Dearman, Rebecca J
AU  - Dearman RJ
FAU - Kimber, Ian
AU  - Kimber I
FAU - Ryan, Cindy A
AU  - Ryan CA
FAU - Gerberick, G Frank
AU  - Gerberick GF
FAU - Lalko, Jon
AU  - Lalko J
FAU - Api, Anne Marie
AU  - Api AM
LA  - eng
PT  - Journal Article
PL  - England
TA  - J Appl Toxicol
JT  - Journal of applied toxicology : JAT
JID - 8109495
RN  - EC 3.1.3.48 (Leukocyte Common Antigens)
SB  - IM
MH  - Animals
MH  - Female
MH  - Leukocyte Common Antigens/*analysis
MH  - *Local Lymph Node Assay
MH  - Lymph Nodes/chemistry/*cytology
MH  - Lymphocyte Activation/drug effects
MH  - Lymphocytes/*chemistry/drug effects
MH  - Mice
MH  - Mice, Inbred CBA
EDAT- 2007/06/02 09:00
MHDA- 2007/10/27 09:00
CRDT- 2007/06/02 09:00
PHST- 2007/06/02 09:00 [pubmed]
PHST- 2007/10/27 09:00 [medline]
PHST- 2007/06/02 09:00 [entrez]
AID - 10.1002/jat.1269 [doi]
PST - ppublish
SO  - J Appl Toxicol. 2007 Sep-Oct;27(5):506-10. doi: 10.1002/jat.1269.